1887

Abstract

SUMMARY

A mutant of 32, defective for deoxythymidine kinase (ATP: thymidine 5′-phosphotransferase EC 2.7.1.21) and therefore unable to incorporate thymidine into DNA has been used to study alterations in thymidine utilization resulting from phage infection. Infection by the T-even phages leads to the rapid incorporation of [H]-thymidine into DNA which results from the induction of deoxythymidine kinase activity as first reported by Hiraga, Igarashi & Yura (1967). No effect was observed following infection of 32 by phages T3, T7, λ, Pl and Mu-1. The T4-induced enzyme differs from the wild-type cell enzyme in several properties including heat stability, deoxycytidine triphosphate stimulation and pH optimum and the ability to induce deoxythymidine kinase can be lost by mutation in a T4 gene () linked to I. T4 mutant-infected 32 cells fail to incorporate [H]-thymidine or 5-bromodeoxyuridine into DNA and the latter property formed the basis for the selection of the phage mutants. The T4 gene is non-essential for growth both in wild type and mutant cells since deoxythymidine monophosphate can be synthesised by the pathway.

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/content/journal/jgv/10.1099/0022-1317-24-1-115
1974-07-01
2021-10-17
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References

  1. Adams M. H. 1959 In Bacteriophages New York: Interscience;
    [Google Scholar]
  2. Benzer S. 1957; The elementary units of heredity. In The Chemical Basis of Heredity70–93 Edited by Mcelroy W. D., Glass B. Baltimore: The Johns Hopkins Press;
    [Google Scholar]
  3. Bresnick E., Thompson U. B. 1965; Properties of deoxythymidine kinase purified from animal tumours. Journal of Biological Chemistry 24:3967–3974
    [Google Scholar]
  4. Chace K. V., Hall D. W. 1973; Isolation of mutants of bacteriophage T4 unable to induce thymidine kinase activity. Journal of Virology 12:343–348
    [Google Scholar]
  5. Dubbs D. R., Kit S. 1964; Isolation and properties of vaccinia mutants deficient in thymidine kinase-inducing activity. Virology 22:214–225
    [Google Scholar]
  6. Greer S. 1960; Studies on ultraviolet irradiation of Escherichia coli containing 5-bromouracil in its DNA. Journal of General Microbiology 22:618–634
    [Google Scholar]
  7. Hall D. H. 1967; Mutants of bacteriophage T4 unable to induce dihydrofolate reductase activity. Proceedings of the National Academy of Sciences of the United States of America 58:584–591
    [Google Scholar]
  8. Hall D. H., Tessman I. 1966; T4 mutants unable to induce deoxycytidylate deaminase activity. Virology 29:339–345
    [Google Scholar]
  9. Hall D. H., Tessman I., Karlstrom O. 1967; Linkage of T4 genes controlling a series of steps in pyrimidine synthesis. Virology 31:442–448
    [Google Scholar]
  10. Hershey A. D., Chase M. 1952; Independent functions of viral protein and nucleic acid in growth of bacteriophage. Journal of General Physiology 36:39–56
    [Google Scholar]
  11. Hiraga S., Igarashi K., Yura T. 1967; A deoxythymidine kinase-deficient mutant of Escherichia coli. I. Isolation and some properties. Biochimica et biophysica acta 145:41–51
    [Google Scholar]
  12. Igarashi K., Hiraga S., Yura T. 1967; A deoxythymidine kinase-deficient mutant of Escherichia coli. II. Mapping and transduction studies with phage 9I80. Genetics 57:643–654
    [Google Scholar]
  13. Johnson J. R., Hall D. H. 1973; Isolation and characterisation of mutants of bacteriophage T4 resistant to folate analogs. Virology 53:413–426
    [Google Scholar]
  14. Kit S., Dubbs D. R., Anken M. 1967; Altered properties of thymidine kinase after infection of mouse fibroblast cells with herpes simplex virus. Journal of Virology 1:238–240
    [Google Scholar]
  15. Krieg D. 1959; A study of gene action in ultraviolet irradiated bacteriophage T4. Virology 8:80–98
    [Google Scholar]
  16. Littlefield J. W., Basilico C. 1966; Infection of thymidine kinase-deficient BHK cells with polyoma virus. Nature, London 211:250–252
    [Google Scholar]
  17. Machattie L. A., Ritchie D. A., Thomas C. A. Jun, Richardson C. C. 1967; Terminal repetition in permuted T2 bacteriophage DNA molecules. Journal of Molecular Biology 23:355–363
    [Google Scholar]
  18. Mahmood N., Lunt M. R. 1972; Biochemical changes during mixed infections with bacteriophages T2 and T4. Journal of General Virology 16:185–198
    [Google Scholar]
  19. Okazaki R., Kornberg A. 1964a; Deoxythymidine kinase of Escherichia coli. I. Purification and some properties of the enzyme. Journal of Biological Chemistry 239:269–274
    [Google Scholar]
  20. Okazaki R., Kornberg A. 1964b; Deoxythymidine kinase of Escherichia coli. II. Kinetics and feedback control. Journal of Biological Chemistry 239:275–284
    [Google Scholar]
  21. Ritchie D. A., Malcolm F. E. 1970; Heat-stable and density mutants of phages Ti, T3 and T7. Journal of General Virology 9:35–43
    [Google Scholar]
  22. Sheinin R. 1966; Studies on the thymidine kinase activity of mouse embryo cells infected with polyoma virus. Virology 28:47–55
    [Google Scholar]
  23. Simon E. H., Tessman I. 1963; Thymidine requiring mutants of phage T4. Proceedings of the National Academy of Sciences of the United States of America 50:526–532
    [Google Scholar]
  24. Stahl F. W., Craseman J. M., Okun L., Fox E., Laird C. 1961; Radiation sensitivity of bacteriophage containing 5-bromodeoxyuridine. Virology 13:98–104
    [Google Scholar]
  25. Tessman L., Greenberg D. B. 1972; Ribonucleotide reductase genes of phage T4: map location of the thioredoxin gene nrdC . Virology 49:337–338
    [Google Scholar]
  26. Warner H. R., Barnes J. E. 1966; Evidence for a dual role for the bacteriophage T4-induced deoxycytidine triphosphate nucleotidohydrolase. Proceedings of the National Academy of Sciences of the United States of America 56:1233–1240
    [Google Scholar]
  27. Yeh Y. C., Tessman I. F. 1972; Control of pyrimidine biosynthesis in phage T4. II. In vitro complementation between ribonucleotide reductase mutants. Virology 47:767–772
    [Google Scholar]
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