The association of the replicase of foot-and-mouth disease virus (FMDV) with cellular membranes was suggested following attempts to produce a soluble enzyme by the use of detergents (Arlinghaus & Polatnick, 1967, 1969; Delagneau, 1971), as described for the purification of poliovirus replicase (Ehrenfeld, Maizel & Summers, 1970). Subsequently, the association with cellular membranes of the replicase and the single-stranded and replicative forms of RNA was reported for poliovirus (Caliguiri & Tamm, 1969, 1970), for Semliki Forest virus (Friedman 1972) and for tobacco mosaic virus (Ralph, Bullivant & Wojcik, 1971). In each case, the replicase-membrane complex contained endogenous RNA template (Arlinghaus & Polatnick, 1969; Caliguiri & Tamm, 1969; Ehrenfeld 1970; Friedman 1972). We show in this communication that FMDV replicase is associated with ribosome-containing membranes and requires glycerol to stabilize its activity during sedimentation in the presence of detergents.


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