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Virus-like particles have been detected in the sap of red clover plants (Trifolium pratense) infected with rugose leaf curl disease and in the salivary glands of the leafhopper vector (Austroagallia torrida), but not in healthy red clover. Some discrepancy in size between the particles isolated from the plant sap and those seen in sections of the salivary glands is attributed to the removal of the outer envelopes of the virus particles during their isolation from plant material.
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