Treatment of purified Semliki Forest virus with neuraminidase released sialic acid, raised the isoelectric point of the virus by 0.35 pH units but had no effect on the infectivity, haemagglutinating (HA) activity or surface antigenic properties and did not alter the electrophoretic mobility of the envelope glycoproteins on polyacrylamide gels. Treatment of virus with a mixture of sugar hydrolases lowered infectivity and HA activity and slightly increased the electrophoretic mobility of the envelope glycoproteins, but had no measurable effect on surface antigenic properties. Treatment with bromelain digested the envelope glycoproteins, destroyed infectivity, HA activity and surface antigenicity and yielded a sub-viral particle containing lipid. Treatment of this particle with phospholipase C produced a core particle indistinguishable by sedimentation analysis from the nucleocapsids found in infected cells.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error