1887

Abstract

SUMMARY

Mutant D1 is the only member of complementation group D of vesicular stomatitis virus (VSV), New Jersey serotype (Pringle, Duncan & Stevenson, 1971). At the non-permissive temperature (39 °C) both virus RNA and structural proteins are synthesized in BHK-21 cells infected with this mutant (Wunner & Pringle, 1972).

Comparison of virus particle and intracellular proteins have shown that polypeptides G and N of D1 grown at 31 °C, and intracellular proteins at 39 °C, have altered electrophoretic mobilities in 10% SDS-polyacrylamide gel corresponding to mol. wt. differences of 3500 and 1000 respectively, yet viability at the permissive temperature was unimpaired. The differences were evident by both continuous and discontinuous SDS-polyacrylamide gel electrophoresis.

Reversion of the phenotype to wild type was accompanied by a restoration of normal mobility to polypeptide N, whereas G retained the abnormal mobility of the mutant. Thus the phenotype is directly related to the altered mobility of N.

The extent of glycosylation of G in mutant D1 appeared to be different from that of group C mutants and wild type.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-23-1-97
1974-04-01
2022-01-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/23/1/JV0230010097.html?itemId=/content/journal/jgv/10.1099/0022-1317-23-1-97&mimeType=html&fmt=ahah

References

  1. Bishop D. H. L., Roy P. 1972; Dissociation of vesicular stomatitis virus and relation of the virion proteins to the viral transcriptase. Journal of Virology 10:234–243
    [Google Scholar]
  2. Cartwright B., Talbot P., Brown F. 1970; The proteins of biologically active sub-units of vesicular stomatitis virus. Journal of General Virology 7:267–272
    [Google Scholar]
  3. Flamand A., Pringle C. R. 1971; The homologies of spontaneous and induced temperature-sensitive mutants of vesicular stomatitis virus isolated in chick embryo and BHK 21 cells. Journal of General Virology 11:81–85
    [Google Scholar]
  4. Helleiner C. H., Wunner W. H. 1971; A simple method for counting 14C- and sH-protein in polyacrylmide gels. Analytical Biochemistry 39:333–338
    [Google Scholar]
  5. Kang C. Y., Prevec L. 1970; Proteins of vesicular stomatitis virus. II. Immunological comparisons of viral antigens. Journal of Virology 6:20–27
    [Google Scholar]
  6. Kelley J. M., Emerson S. U., Wagner R. R. 1972; The glycoprotein of vesicular stomatitis virus is the antigen that gives rise to and reacts with neutralizing antibody. Journal of Virology 10:1231–1235
    [Google Scholar]
  7. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  8. Macpherson I. A., Stoker M. G. P. 1962; Polyoma transformation of hamster cell clones - an investigation of genetic factors affecting cell competence. Virology 16:147–151
    [Google Scholar]
  9. Pringle C. R. 1970; Genetic characteristics of conditional lethal mutants of vesicular stomatitis virus induced by 5-fluorouracil, 5-azacytidine and ethyl methane sulfonate. Journal of Virology 5:559–567
    [Google Scholar]
  10. Pringle C. R., Duncan I. B., Stevenson M. 1971; Isolation and characterisation of temperature-sensitive mutants of vesicular stomatitis virus, New Jersey serotype. Journal of Virology 8:836–841
    [Google Scholar]
  11. Pringle C. R., Wunner W. H. 1973; Genetic and physiological properties of temperature-sensitive mutants of Cocal virus (VSV Cocal). Journal of Virology 12:677–683
    [Google Scholar]
  12. Roy P., Bishop D. H. L. 1973; Initiation and direction of RNA transcription by vesicular stomatitis virus virion transcriptase. Journal of Virology 11:487–501
    [Google Scholar]
  13. Sokol F., Clark F. H. 1973; Phosphoproteins, structural components of rhabdoviruses. Virology 52:246–63
    [Google Scholar]
  14. Wunner W. H., Pringle C. R. 1972a; Protein synthesis in BHK 21 cells infected with vesicular stomatitis virus. I. ts mutants of the Indiana serotype. Virology 48:104–111
    [Google Scholar]
  15. Wunner W. H., Pringle C. R. 1972b; Protein synthesis in BHK 21 cells infected with vesicular stomatitis virus. II. ts mutants of the New Jersey serotype. Virology 50:250–253
    [Google Scholar]
  16. Wunner W. H., Pringle C. R. 1972c; Comparison of structural polypeptides from vesicular stomatitis virus (Indiana and New Jersey serotypes) and Cocal virus. Journal of General Virology 16:1–10
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-23-1-97
Loading
/content/journal/jgv/10.1099/0022-1317-23-1-97
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error