Relationship of Cytotoxicity and Interferon-inducing Activity of Polyriboinosinic Acid. Polyribocytidylic Acid to the Molecular Weights of the Homopolymers
The molecular size of poly I.poly C required for lysis of interferon-treated L cells was the same as the size required for induction of interferon in these cells. The size requirement for both interferon induction and toxicity for interferon-treated L cells resided in the poly I strand, while variations of the poly C strand size were without effect on interferon induction or cell lysis.
The size of poly I.poly C required to induce interferon in primed L cells or primed primary rabbit kidney cells was the same as the size required to induce interferon in normal L cells or normal rabbit kidney cells. Again, these requirements proved more stringent for the poly I strand than for the poly C strand. These data suggest that both primed and normal cells recognize the same molecular ‘trigger’ for interferon induction.
BlackD. R.,
EcksteinF.,
De ClercqE.,
MeriGanT. C.1973; Studies on the toxicity and antiviral activity of various polynucleotides. Antimicrobial Agents and Chemotherapy 3:198–206
CarterW. A.,
PithaP. M.,
MarshallL. W.,
TazawaI.,
TazawaS.,
TS’OP. O. P.1972; Structural requirements of the rIn.rCn complex for induction of human interferon. Journal of Molecular Biology 70:567–587
De ClercqE.,
StewartW. E.II,
De SomerP.1973a; Studies on the mechanism of the priming effect of interferon on interferon production by cell cultures exposed to poly(rI). poly(rC). Infection and Immunity 8:309–316
De ClercqE.,
StewartW. E.II,
De SomerP.1973b; Poly(rI) more important than poly(rC) in the interferon induction process by poly(rI). Poly(rC). Virology 54:278–282
DianzaniF.,
CantagalliP.,
CagnoniS.,
RitaG.1968; Effect of DEAE-dextran on production of interferon induced by synthetic double-stranded RNA in L cell cultures. Proceedings of the Society for Experimental Biology and Medicine 128:708–710
FalcoffR.,
FalcoffE.,
CatinotI.1970; Interferon induction by and infectivity of ultraviolet-irradiated double and multistranded viral RNAs. Biochimica et biophysica acta 217:195–196
LampsonG. P.,
FieldA. K.,
TytellA. A.,
NemesM. M.,
HillemanM. R.1970; Relationship of molecular size of rIn: rCn (poly I: C) to induction of interferon and host resistance. Proceedings of the Society for Experimental Biology and Medicine 135:911–916
LampsonG. P.,
NemesM. M.,
FieldA. K.,
TytellA. A.,
HillemanM. R.1972; The effect of altering the size of poly(C) on the toxicity and antigenicity of poly I: C. Proceedings of the Society for Experimental Biology and Medicine 141:1068–1072
MohrS. J.,
BrownD. G.,
CoffeyD. S.1972; Size requirement of polyinosinic acid for DNA synthesis, viral resistance, and increased survival of leukaemic mice. Nature New Biology 240:250–252
NiblackJ. F.,
MccrearyM. B.1971; Relationship of biological activities of poly I. poly C to homopolymer molecular weights. Nature New Biology 233:52–53
RosztoczyI.1971; Interferon induction by polyinosinic. polycytidylic acid in interferon pretreated L cells in absence of DEAE-dextran. Acta Virologica 15:431
RosztoczyI.,
MecsI.1970; Enhancement of interferon synthesis by polyinosinic. polycytidylic acid in L cells pretreated with interferon. Acta virologica 14:398–400
StewartW. E.II,
De ClercqE.,
BilliauA.,
DesmyterJ.,
De SomerP.1972a; Increased susceptibility of cells treated with interferon to the toxicity of polyriboinosinic. polyribocytidylic acid. Proceedings of the National Academy of Sciences of the United States of America 69:1851–1854
StewartW. E.II,
De ClercqE.,
De SomerP.1972c; Cellular alteration by interferon: a virus-free system for assaying interferon. Journal of Virology 10:896–901
StewartW. E.II,
De ClercqE.,
De SomerP.1973a; Specificity of interferon-induced enhancement of toxicity for double-stranded ribonucleic acids. Journal of General Virology 18:237–246
StewartW. E.II,
De ClercqE.,
De SomerP.,
BergK.,
OgburnC. A.,
PauckerK.1973b; Comparative antiviral and non-antiviral activities of highly purified interferon preparations. Nature, London 246:141–143
StewartW. E.II,
GosserI. B.,
LockartR. Z.Jun1971b; Distinguishing characteristics of the interferon responses of primary and continuous mouse cell cultures. Journal of General Virology 13:35–50
StewartW. E.II,
GosserI. B.,
LockartR. Z.Jun1972b; The effect of priming with interferon on interferon production by two lines of L cells. Journal of General Virology 15:85–87
TytellA. A.,
LampsonG. P.,
FieldA. K.,
NemesM. M.,
HillemanM. R.1970; Influence of size of individual homopolynucleotides on the physical and biological properties of complexed rIn:rCn (poly I:C). Proceedings of the Society for Experimental Biology and Medicine 135:917–920
Relationship of Cytotoxicity and Interferon-inducing Activity of Polyriboinosinic Acid. Polyribocytidylic Acid to the Molecular Weights of the Homopolymers