1887

Abstract

SUMMARY

CV1, BSC1, Vero, and primary monkey kidney cells were seeded either at low cell density and infected with poliovirus while growing, or at high cell density and infected under conditions of contact inhibition. Virus RNA synthesis and virus production were delayed in the cells seeded at high cell density, although neither adsorption nor uncoating of the parental virus particles were altered. In spite of the delay, however, virus RNA synthesis, once initiated, proceeded in the same fashion and at the same rate in both growing and resting cells. This implies that one of the first events following uncoating of the infecting virus was temporarily retarded in resting cells. There are indications that this delay might be due to a lag in the formation of parental virus polyribosomes.

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/content/journal/jgv/10.1099/0022-1317-23-1-73
1974-04-01
2024-04-19
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References

  1. Abercrombie M., Ambrose E. J. 1962; The surface properties of cancer cells: A review. Cancer Research 22:525–548
    [Google Scholar]
  2. Baltimore D., Girard M., Darnell J. E. 1966; Aspects of the synthesis of poliovirus RNA and the formation of virus particles. Virology 29:179–189
    [Google Scholar]
  3. Berwald Y., Sachs L. 1965; In vitro transformation of normal cells to tumor cells by carcinogenic hydrocarbons. Journal of the National Cancer Institute 35:641–662
    [Google Scholar]
  4. Bienz K., Egger D., Wolff D. A. 1973; Virus replication, cytopathology and lysosomal enzyme response of mitotic and interphase Hep. 2 cells infected with poliovirus. Journal of Virology 11:565–574
    [Google Scholar]
  5. Cooper P. D. 1966; The inhibition of poliovirus growth by actinomycin D and the prevention of the inhibition by pretreatment of the cells with serum or insulin. Virology 28:663–678
    [Google Scholar]
  6. Doyle M., Holland J. J. 1972; Virus induced interference inheterologously infected cells. Journal of Virology 9:22–28
    [Google Scholar]
  7. Dulbecco R. 1963; Transformation of cells in vitro by viruses. Science, New York 142:932–936
    [Google Scholar]
  8. Fiszman M., Reynier M., Bucchini D., Girard M. 1972; Thermosensitive block of the Sabin strain of poliovirus type 1. Journal of Virology 10:1143–1151
    [Google Scholar]
  9. Grado C., Fischer S., Contreras G. 1965; The inhibition by actinomycin D of poliovirus multiplication in Hep. 2 cells. Virology 27:623–625
    [Google Scholar]
  10. Hahn J., Fogh J. 1970; Increased resistance of SV40 transformed human amnion cells to poliovirus infection. Archiv für die gesamte Virusforschung 29:343–360
    [Google Scholar]
  11. Holland J. J. 1962; Inhibition of DNA primed RNA synthesis during poliovirus infection of human cells. Biochemical and Biophysical Research Communications 9:556–562
    [Google Scholar]
  12. Johnson T. C., Holland J. J. 1965; Ribonucleic acid and protein synthesis in mitotic HeLa cells. Journal of Cell Biology 27:565–574
    [Google Scholar]
  13. Joklik W., Darnell J. E. 1961; The adsorption and early fate of purified poliovirus in HeLa cells. Virology 13:439–447
    [Google Scholar]
  14. Lake R. S., Winkler D. C., Ludwig U. H. 1970; Delay of Mengovirus induced cytopathology in mitotic L cells. Journal of Virology 5:262–263
    [Google Scholar]
  15. Mccormick W., Penman S. 1968; Replication of Mengovirus in HeLa cells preinfected with non replicating poliovirus. Journal of Virology 2:859–864
    [Google Scholar]
  16. Manteuil S., Pages J., Stehelin D., Girard M. 1973; Replication of Simian Virus 40 deoxyribonucleic acid: Analysis of the one step growth cycle. Journal of Virology 11:98–106
    [Google Scholar]
  17. Reich E., Franklin R. M., Shatkin A. J., Tatum E. L. 1961; Effect of actinomycin D on cellular nucleic acid synthesis and virus production. Science, New York 134:556–557
    [Google Scholar]
  18. Reich E., Franklin R. M., Shatkin A. J., Tatum E. L. 1962; Action of actinomycin D on animal cells and viruses. Proceedings of the National Academy of Sciences of the United States of America 48:1238–1245
    [Google Scholar]
  19. Salzman N. P., Lockart R. Z., Sebring E. D. 1959; Alteration in HeLa cell metabolism resulting from poliovirus infection. Virology 9:244–259
    [Google Scholar]
  20. Schaffer F. L., Gordon M. 1966; Differential inhibitory effects of actinomycin D among strains of poliovirus. Journal of Bacteriology 91:2309–2316
    [Google Scholar]
  21. Shatkin A. J. 1962; Actinomycin inhibition of RNA synthesis and poliovirus infection of HeLa cells. Biochimica et biophysica acta 61:310–313
    [Google Scholar]
  22. Simon E. H. 1961; Evidence for the non participation of DNA in viral RNA synthesis. Virology 13:105–118
    [Google Scholar]
  23. Stanners C. P., Becker H. 1971; Control of macromolecular synthesis in proliferating and resting Syrian hamster cells in monolayer culture. I. Ribosomes function. Journal of Cellular Physiology 77:31–42
    [Google Scholar]
  24. Thorne H. V. 1973; Cyclic variation in susceptibility of Balb-c 3T3 cells to polyoma virus. Journal of General Virology 18:163–169
    [Google Scholar]
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