Recombinant and parental poliovirus particles were indistinguishable by ratezonal and isopycnic sedimentation, and by u.v. inactivation. Sensitive selective procedures, applied to ts+ recombinants to detect genetic segregation of one parent, failed to reveal any. Poliovirus genetic recombinant particles thus appear to be conventional virus particles; their significance for recombination mechanisms is discussed. Sensitivity to the growth inhibitor 2-(3-chloro-p-tolyl)-5-ethyl-1,3,4-oxadiazole is shown to depend on a product of the structural protein gene.
BéchetJ. M.1972; Isolement de la chaȋne ‘moins’ d’acide ribonucléique du virus de l’encephalcmyocardite del a souris et étude de son infectivité. Compte rendu hebdomadaire des séancees de l’ Académie des sciences, Paris, Serie D 274:1761–1764
CooperP. D.,
GeisslerE.,
ScottiP. D.,
TannockG. A.1971; Further characterisation of the genetic map of poliovirus temperature-sensitive mutants. In Strategy of the Viral Genome, Ciba Foundation Symposium pp 75–100 Edited by
WolstenholmeG. E.,
Maeve O’ConnorW.
London: Churchill Livingstone;
CooperP. D.,
WentworthB. B.,
MccahonD.1970b; Guanidine inhibition of poliovirus: a dependence of viral RNA synthesis on the configuration of structural protein. Virology 40:486–493
SilberR.,
MalathiV.G.,
HurwitzJ.1972; Purification and properties of bacteriophage T4-induced RNA ligase. Proceedings of the National Academy of Sciences of the United States of America 69:3009–3013
Steiner-PryorA.,
CooperP. D.1973; Temperature-sensitive poliovirus mutants defective in repression of host protein are also defective in structural protein. Journal of General Virology 21:215–225
SummersD. F.,
MaizelJ. V.1971; Determination of the gene sequence of poliovirus with pactamycin. Proceedings of the National Academy of Sciences of the United States of America 68:2852–2856
TaberR.,
RekoshD.,
BaltimoreD.1971; Effect of pactamycin on synthesis of poliovirus proteins: a method for genetic mapping. Journal of Virology 8:395–401
YamaziY.,
TakahashiM.,
TodomeY.1970; Inhibition of poliovirus by effect of a methylthiopyrimidine derivative. Proceedings of the Society for Experimental Biology and Medicine 133:674–677