On the Nature of Poliovirus Genetic Recombinants Free

Abstract

SUMMARY

Recombinant and parental poliovirus particles were indistinguishable by ratezonal and isopycnic sedimentation, and by u.v. inactivation. Sensitive selective procedures, applied to recombinants to detect genetic segregation of one parent, failed to reveal any. Poliovirus genetic recombinant particles thus appear to be conventional virus particles; their significance for recombination mechanisms is discussed. Sensitivity to the growth inhibitor 2-(3-chloro--tolyl)-5-ethyl-1,3,4-oxadiazole is shown to depend on a product of the structural protein gene.

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1974-04-01
2024-03-29
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References

  1. Béchet J. M. 1972; Isolement de la chaȋne ‘moins’ d’acide ribonucléique du virus de l’encephalcmyocardite del a souris et étude de son infectivité. Compte rendu hebdomadaire des séancees de l’ Académie des sciences, Paris, Serie D 274:1761–1764
    [Google Scholar]
  2. Cooper P. D. 1964; The mutation of poliovirus by 5-fluorouracil. Virology 22:186–192
    [Google Scholar]
  3. Cooper P. D. 1968; A genetic map of poliovirus temperature-sensitive mutants. Virology 35:584–596
    [Google Scholar]
  4. Cooper P. D. 1969; The genetic analysis of poliovirus. In Biochemistry of Viruses pp 177–218 Edited by Levy H. B. New York: Marcel Dekker;
    [Google Scholar]
  5. Cooper P. D., Geissler E., Scotti P. D., Tannock G. A. 1971; Further characterisation of the genetic map of poliovirus temperature-sensitive mutants. In Strategy of the Viral Genome, Ciba Foundation Symposium pp 75–100 Edited by Wolstenholme G. E., Maeve O’Connor W. London: Churchill Livingstone;
    [Google Scholar]
  6. Cooper P. D., Stanček D., Summers D. F. 1970a; Synthesis of double-stranded RNA by poliovirus temperature-sensitive mutants. Virology 40:971–977
    [Google Scholar]
  7. Cooper P. D., Steiner-Pryor A., Wright P. J. 1973; A proposed regulator for poliovirus: the equestron. Intervirology 1:1–10
    [Google Scholar]
  8. Cooper P. D., Wentworth B. B., Mccahon D. 1970b; Guanidine inhibition of poliovirus: a dependence of viral RNA synthesis on the configuration of structural protein. Virology 40:486–493
    [Google Scholar]
  9. Cranston J., Malathi V. G., Silber R. 1973; Further studies on RNA ligase. Federation Proceedings Abstracts 32:498
    [Google Scholar]
  10. Hotchkiss R. D. 1971; Towards a general theory of genetic recombination in DNA. Advances in Genetics 16:325–348
    [Google Scholar]
  11. Ӧberg B., Philipson L. 1971; Replicative structures of poliovirus RNA in vivo. Journal of Molecular Biology 58:725–737
    [Google Scholar]
  12. Silber R., Malathi V.G., Hurwitz J. 1972; Purification and properties of bacteriophage T4-induced RNA ligase. Proceedings of the National Academy of Sciences of the United States of America 69:3009–3013
    [Google Scholar]
  13. Steiner-Pryor A., Cooper P. D. 1973; Temperature-sensitive poliovirus mutants defective in repression of host protein are also defective in structural protein. Journal of General Virology 21:215–225
    [Google Scholar]
  14. Summers D. F., Maizel J. V. 1971; Determination of the gene sequence of poliovirus with pactamycin. Proceedings of the National Academy of Sciences of the United States of America 68:2852–2856
    [Google Scholar]
  15. Taber R., Rekosh D., Baltimore D. 1971; Effect of pactamycin on synthesis of poliovirus proteins: a method for genetic mapping. Journal of Virology 8:395–401
    [Google Scholar]
  16. Wright P. J., Cooper P. D. 1974; Poliovirus proteins associated with ribosomal structures in infected cells. Virology (in the press)
    [Google Scholar]
  17. Yamazi Y., Takahashi M., Todome Y. 1970; Inhibition of poliovirus by effect of a methylthiopyrimidine derivative. Proceedings of the Society for Experimental Biology and Medicine 133:674–677
    [Google Scholar]
  18. Závadová Z. 1971; Host cell repair of vaccinia virus and of double-stranded RNA of encephalomyocarditis virus. Nature New Biology 233:123
    [Google Scholar]
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