1887

Abstract

SUMMARY

Human serum was fractionated by ultracentrifuging and the non-specific inhibitory activity for the togaviruses, Japanese encephalitis virus and rubella virus, was found chiefly in the very low density lipoprotein (VLDL) and low density lipoprotein (LDL) fractions. The specific inhibitory activity per mg protein or lipid of each lipoprotein fraction was greater for Japanese encephalitis virus. Japanese encephalitis virus was significantly more sensitive to VLDL than LDL whereas rubella virus was equally sensitive to either.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-23-1-113
1974-04-01
2024-12-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/23/1/JV0230010113.html?itemId=/content/journal/jgv/10.1099/0022-1317-23-1-113&mimeType=html&fmt=ahah

References

  1. Bidwell D. E., Mills G. L. 1968; Serum non-specific inhibitors of arbovirus haemagglutination. Journal of Comparative Pathology 78:469–476
    [Google Scholar]
  2. Chang T., Weinstein L. 1972; Studies of non-specific inhibition of rubella virus and isolation and identification of inhibitor. American Journal of Medical Sciences 263:233–239
    [Google Scholar]
  3. Clarke D. H., Casals J. 1958; Techniques for haemagglutination and haemagglutination inhibition with arthropod-borne viruses. American Journal of Tropical Medicine and Hygiene 7:561–573
    [Google Scholar]
  4. Feldman H. A. 1968; Removal by Heparin-MnCl2 of non-specific rubella haemagglutination inhibitor. Proceedings of the Society for Experimental Biology and Medicine 127:570–573
    [Google Scholar]
  5. Frings C. S., Fendley T. W., Dunn R. T., Queen C. A. 1972; Improved determination of total serum lipids by sulfo-phosphovanillin reaction. Clinical Chemistry 18:673–674
    [Google Scholar]
  6. Gorman B. 1970; Lipid inhibitors of arbovirus haemagglutination. Journal of General Virology 6:305–313
    [Google Scholar]
  7. Hatch F. T., Lees R. S. 1968; Practical methods for lipoprotein analysis. Advances in Lipid Research 6:1–68
    [Google Scholar]
  8. Hatch F. T., Lindgren S. T., Adamson G. L., Jensen L. C., Wong A. W., Levy R. I. 1973; Quantitative agarose gel electrophoresis of plasma lipoproteins: a simple technique and two methods for standardization. Journal of Laboratory and Clinical Medicine 81:946–960
    [Google Scholar]
  9. Haukenes G., Aasen J. 1971; Rubella haemagglutination inhibitors: their separation from antibodies. Acta pathologica et microbiologica scandinavica Section B 79:679–685
    [Google Scholar]
  10. Laufs R., Thomssen R. 1968; Inhibition der Rubellavirus-hämagglutination. I. Eigenschaften eines in fetalem Kälberserum vorkommenden Inhibitors der Rubellavirus-Hämagglutination in vitro. Archiv für die gesamte Virusforschung 24:181–191
    [Google Scholar]
  11. Liebhaber H. 1970; Measurement of rubella antibody by haemagglutination inhibition. I. Variables affecting rubella haemagglutination. Journal of Immunology 104:818–825
    [Google Scholar]
  12. Lowry O. H., Rosebrough W. O., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  13. Nicoli J., Acker P. 1965; Les inhibiteurs sériques de l’hémagglutination par les arbovirus. Annales de l’Institut Pasteur, Paris 108:22–32
    [Google Scholar]
  14. Porterfield J. S., Rowe C. E. 1960; Haemagglutination with arthropod-borne viruses and its inhibition by certain phospholipids. Virology II:765–770
    [Google Scholar]
  15. Salimen A. 1962; Chemistry of non-specific inhibitors of haemagglutination by arthropod-borne viruses. Virology 16:201–203
    [Google Scholar]
  16. Shortridge K. F., Biddle F., Pepper D. S. 1972; Rubella virus non-specific haemagglutination inhibitor: evidence for the role of glycolipid bound to low density (β) lipoprotein. Clinica chimica acta 42:285–294
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-23-1-113
Loading
/content/journal/jgv/10.1099/0022-1317-23-1-113
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error