1887

Abstract

SUMMARY

The capsid polypeptides of virus S (PsV-S) and virus F (PsV-F) were examined by SDS-polyacrylamide gel electrophoresis. PsV-S contained one major (S2) and one minor (S1) polypeptide with mol. wt. 42500 and 55500, while PsV-F contained one major (F2) and one minor (F1) polypeptide with mol. wt. 47000 and 59000, respectively. Mol. wt. of S2 and F2 polypeptides calculated from amino acid analyses were 41500 and 48000, respectively. PsV-S capsid was estimated to contain 120 molecules of polypeptide S2 and 1 molecule of polypeptide S1.

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/content/journal/jgv/10.1099/0022-1317-22-3-441
1974-03-01
2024-04-19
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References

  1. Adler J. P., Mackenzie D. W. 1972; Fungal viruses: intrahyphal localization of Penicillium stoloniferum viruses by fluorescent antibody. Abstracts of the Annual Meeting of the American Society for Microbiology 72:68
    [Google Scholar]
  2. Banks G. T., Buck K. W., Chain E. B., Himmelweit F., Marks J. E., Tyler J. M., Hollings M., Last F. T., Stone O. M. 1968; Viruses in fungi and interferon stimulation. Nature, London 218:542–545
    [Google Scholar]
  3. Bozarth R. F., Wood H. A., Mandelbrot A. 1971; The Penicillium stoloniferum virus complex; two similar double-stranded RNA virus-like particles in a single cell. Virology 45:516–523
    [Google Scholar]
  4. Buck K. W., Kempson-Jones G. F. 1970; Three types of virus particle in Penicillium stoioniferum. Nature, London 225:945–946
    [Google Scholar]
  5. Buck K. W., Kempson-Jones G. F. 1973; Biophysical properties of Penicillium stoloniferum virus S. Journal of General Virology 18:223–235
    [Google Scholar]
  6. Goodwin T. W., Morton R. A. 1964; The spectrophotometric determination of tyrosine and tryptophan in proteins. Biochemical Journal 40:628–632
    [Google Scholar]
  7. Hirs C. H. W. 1967; Determination of cystine as cysteic acid. In Methods in Enzymology vol 11: p 59–62
    [Google Scholar]
  8. Symons R. H., Rees M. W., Short M. N., Markham R. 1963; Relationship between the ribonucleic acid and protein of some plant viruses. Journal of Molecular Biology 6:1–15
    [Google Scholar]
  9. Thomas A. J. 1970 In Automation, Mechanisation and Data Handling in Microbiology vol 4 p 107 Edited by Baillie A., Gilbert R. J. London: Academic Press;
    [Google Scholar]
  10. Weber K., Osborn N. 1969; The reliability of molecular weight determinations by dodecyl-sulphate polyacrylamide gel electrophoresis. Journal o f Biological Chemistry 244:4406–4412
    [Google Scholar]
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