1887

Abstract

SUMMARY

Phage ST-1 and a host range mutant, ST-1 h, the only members of the X174 class of bacteriophage capable of growth on male strains of , were characterized with respect to their growth requirements and physical properties. These phage were found to be separable on the basis of host range and antigenic properties but not by density or morphology. Phage ST-1 showed a strict requirement for the presence of divalent cations in order for growth to occur. This requirement was shown to be at the attachment stage in the infection process. Maximal adsorption kinetics occurred in the presence of 0.016 -CaCl and both ST-1 and ST-1 h showed a greater response to divalent cations than did X174. The interaction of these phage with isolated bacterial cell walls showed that the adsorption site involved the lipid layers but not the muramic acid portion of the cell wall. The growth of phage ST-1 on the mutant bacterial strain w-3110/1 revealed a strict requirement for the presence of calcium at a period after attachment.

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/content/journal/jgv/10.1099/0022-1317-22-3-411
1974-03-01
2022-08-18
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References

  1. Bayer M. E., Starkey T. W. 1972; The adsorption of bacteriophage ϕ X174 and its interaction with Escherichia coli a kinetic and morphological study. Virology 49:236–259
    [Google Scholar]
  2. Bleichrodt J. F., Berends-Van Abkoude E. R. 1968; Bacteriophages related to ϕ X174 showing a transition between two forms with different heat sensitivity and adsorption behavior. Virology 34:366–370
    [Google Scholar]
  3. Bowes J. M., Dowell C. E. 1970; Some characteristics and growth parameters of the bacteriophage ST-1. Presented at the 70th Annual Meeting, American Society for Microbiology Boston; Massachusetts:
    [Google Scholar]
  4. Bradley D. E. 1970; A comparative study of some properties of the ϕ X174 type bacteriophages. Canadian Journal of Microbiology 16:969–971
    [Google Scholar]
  5. Bradley D. E. 1964; The structure of some bacteriophages associated with male strains of Escherichia coli. Journal of General Microbiology 35:471–482
    [Google Scholar]
  6. Brown D. T., Mackenzie J. M., Bayer M. E. 1971; Mode of host cell penetration by bacteriophage ϕ X174. Journal of Virology 7:836–846
    [Google Scholar]
  7. Dalgarno L., Sinsheimer R. L. 1968; Process of infection with bacteriophage ϕ X174. XXIV. New type of temperature-sensitive mutant. Journal of Virology 2:822–829
    [Google Scholar]
  8. Ellis E. L., Delbruck M. 1939; The growth of bacteriophage. Journal of General Physiology 22:365–384
    [Google Scholar]
  9. Fiers W., Lepoutre L., Vandendriessche L. 1965; Studies on the bacteriophage MS2.1. Distribution of purine sequences in the viral RNA and in yeast RNA. Journal of Molecular Biology 13:432–450
    [Google Scholar]
  10. Fujimura R., Kaesberg P. 1962; The adsorption of bacteriophage ϕ X174 to its host. Biophysics Journal 2:433–449
    [Google Scholar]
  11. Hershey A. D., Chase M. 1952; Independent functions of viral protein and nucleic acid in growth of bacteriophage. Journal of General Physiology 36:39–56
    [Google Scholar]
  12. Newbold J. E., Sinsheimer R. L. 1970; The process of infection with bacteriophage ϕ X174. XXXII. Early steps in the infection process: Attachment, eclipse and DNA penetration. Journal of Molecular Biology 49:49–66
    [Google Scholar]
  13. Sinsheimer R. L. 1959; Purification and properties of bacteriophage ϕ X174. Journal of Molecular Biology 1:37–42
    [Google Scholar]
  14. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology 40:734–744
    [Google Scholar]
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