We have studied some properties of Nudaurelia capensis β virus, a non-occluded isometric RNA virus isolated from the infected larvae of the Pine Emperor moth, Nudaurelia cytherea capensis (Lepidoptera: Saturniidae). The virus was degraded by treatment with formic acid, and the mol. wt. of the coat protein was estimated by polyacrylamide gel electrophoresis to be between 60000 and 62000. The virus contains 11% single-stranded RNA with a nucleotide composition of 28% guanylic acid, 23% uridylic acid, 25% cytidylic acid, and 24% adenylic acid. The cryptogram of the virus is R/1; 1.8/11; S/S; I/O.
HedrickJ. L.,
SmithA. J.1968; Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis. Archives of Biochemistry and Biophysics 126:155–164
HendryD. A.1969; Biophysical studies of two viruses: cucumber mosiac virus and Nudaurelia non-occluded virus. M.Sc. dissertation University of Stellenbosch;
HendryD. A.,
BekkerM. F.,
Van RegenmortelM. H. V.1968; A nonoccluded virus of the pine emperor moth, Nudaurelia cytherea capensis Stoll. South African Medical Journal 42:117 (abst)
JuckesI. R. M.,
LongworthJ. F.,
ReinganumC.1973; A serological comparison of some nonoccluded insect viruses. Journal of Invertebrate Pathology 21:119–120
LongworthJ. F.,
PayneC. C.,
MacleodR.1973; Studies on a virus from Gonometa podocarpi (Lepidoptera: Lasiocampidae). Journal of General Virology 18:119–125
PolsonA.,
StannardL.,
TripconeyD.1970; The use of haemocyanin to determine the molecular weight of Nudaurelia cytherea capensis β virus by direct particle counting. Virology 41:680–687
SchaferW.,
LangeJ.,
BolognesiD. P.,
NoronhaF. D.,
PostJ. E.,
RichardC. G.1971; Isolation and characterisation of two group specific antigens from feline leukemia virus. Virology 44:73–82
ShapiroA. L.,
VinuelaE.,
MaizelJ. V.1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochemical and Biophysical Research Communications 28:815–820