1887

Abstract

SUMMARY

The arginine analogue canavanine strongly inhibited the replication of herpes simplex virus in BSC-1 cells. Virus absorption and entry of the virus DNA into the cell nucleus were unaffected. However, changes qualitatively similar to those produced by arginine deprivation did occur. There was little inhibition of protein synthesis but the transport of proteins from cytoplasm to nucleus was substantially reduced. Virus DNA synthesis which was extremely sensitive early in the growth cycle was little affected at late times although virus maturation was still prevented. Possible modes of action of the drug are discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-22-3-319
1974-03-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/22/3/JV0220030319.html?itemId=/content/journal/jgv/10.1099/0022-1317-22-3-319&mimeType=html&fmt=ahah

References

  1. Becker Y., Olshevsky U., Levitt J. 1967; The role of arginine in the replication of herpes simplex virus. Journal of General Virology 1:471–478
    [Google Scholar]
  2. Bollum F. J. 1959; Thermal conversion of non-priming deoxyribonucleic acid to primer. Journal of Biological Chemistry 234:2733–2734
    [Google Scholar]
  3. Bonifas V. H., Schlesinger R. W. 1959; Nutrient requirements for plaque formation by adenovirus. Federation Proceedings 18:560
    [Google Scholar]
  4. Courtney R. J., Mccombs R. M., Benyesh-Melnick M. 1970; Antigens specified by herpesviruses. I. Effect of arginine deprivation in antigen synthesis. Virology 40:379–386
    [Google Scholar]
  5. Courtney R. J., Mccombs R. M., Benyesh-Melnick M. 1971; Antigens specified by herpesviruses. II Effect of arginine deprivation on the synthesis of cytoplasmic and nuclear proteins. Virology 43:356–365
    [Google Scholar]
  6. Gfeller E., Russell D. H. 1970; Distribution of [3H] polyamines in a Xenopus laevis liver cell line. Anatomical Records 166:306
    [Google Scholar]
  7. Gibson W., Roizman B. 1971; Compartmentalization of spermine and spermidine in the herpes simplex virion. Proceedings of the National Academy of Sciences of the United States of America 68:2818–2821
    [Google Scholar]
  8. Inglis V. B. M. 1968; Requirements of arginine for the replication of herpesviruses. Journal of General Virology 3:9–17
    [Google Scholar]
  9. Mark G. E., Kaplan A. S. 1971; Synthesis of proteins in cells infected with herpesviruses. VII. Lack of migration of structural viral proteins to the nucleus of arginine-deprived cells. Virology 45:53–60
    [Google Scholar]
  10. Morris D. R., Pardee A. B. 1966; Multiple pathways of putrescine biosynthesis in Escherichia coli. Journal of Biological Chemistry 241:3129–3135
    [Google Scholar]
  11. Raška K., Prage L., Schlesinger R. W. 1972; Effects of arginine starvation on macromolecular synthesis in infection with type 2 adenovirus. II. Synthesis of virus-specific RNA and DNA. Virology 48:472–484
    [Google Scholar]
  12. Roizman B., Spring S. B., Roane P. R. 1967; Cellular compartmentalization of herpesvirus antigens during viral replication. Journal of Virology 1:181–192
    [Google Scholar]
  13. Russell D. H. 1970; Putrescine and spermidine biosynthesis in growth and development. Annals of the New York Academy of Science 171:772–782
    [Google Scholar]
  14. Schactele C. F., Rogers P. 1968; Mechanism of canavanine death in Escherichia coli. I. Effect of canavanine on macromolecular synthesis. Journal of Molecular Biology 33:843–860
    [Google Scholar]
  15. Schactele C. F., Anderson D. L., Rogers P. 1968; Mechanism of canavanine death in Escherichia coli. II. Membrane-bound canavanyl-protein and nuclear disruption. Journal of Molecular Biology 33:861–872
    [Google Scholar]
  16. Sharon N. 1966; Stimulation of herpes simplex virus propagation in Wish cells by arginine. International Congress of Microbiology 9th Abstract 508
    [Google Scholar]
  17. Tabor H., Tabor C. W. 1964; Spermidine, spermine and related amines. Pharmacological Reviews 16:245–300
    [Google Scholar]
  18. Tankersley R. W. Jun 1964; Amino acid requirements of herpes simplex virus in human cells. Journal of Bacteriology 87:609–613
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-22-3-319
Loading
/content/journal/jgv/10.1099/0022-1317-22-3-319
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error