Non-specific Serum Inhibitors of Activity of Haemagglutinins of Rabies and Vesicular Stomatitis Viruses Free

Abstract

SUMMARY

A pooled normal human serum at up to about 1:4000 dilution inhibited haemagglutination by rabies and vesicular stomatitis viruses. Serum lipoproteins were found to carry almost all of the inhibitory activity, which specifically resided in the phospholipids of these lipoproteins. Commercially available phospholipids and cholesterol were also effective inhibitors of virus haemagglutination. The inhibitory activity of the mixture of cholesterol and lecithin tested against the haemagglutinins of vesicular stomatitis virus, rabies virus and an arbovirus (LaCrosse strain of California arbovirus group) were 2 to 8 times higher than that for the lipids alone, whereas the inhibitory activity of the mixture of cholesterol and palmitic acid was not higher than that of cholesterol alone. Adsorption with kaolin, as well as fractionation by DEAE-cellulose chromatography, effectively separated the non-specific inhibitors from specific antibodies contained in burro anti-rabies virus serum.

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/content/journal/jgv/10.1099/0022-1317-22-3-309
1974-03-01
2024-03-28
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References

  1. Altemeier W. A., Mundon F. K., Top F. H., Russell P. K. 1970; Method for extracting viral hemagglutination-inhibiting antibodies from the nonspecific inhibitors of serum. Applied Microbiology 19:785–790
    [Google Scholar]
  2. Arstila P., Halonen P. E., Salmi A. 1969; Hemagglutinin of vesicular stomatitis virus. Archiv für die gesamte Virusforschung 27:198–208
    [Google Scholar]
  3. Bidwell D. E., Mills G. L. 1968; Serum non-specific inhibitors of arbovirus haemagglutination. Journal of Comparative Pathology 78:469–476
    [Google Scholar]
  4. Chappell W. A., Halonen P. E., Toole R. F., Calisher C. H., Chester L. 1969; Preparation of LaCrosse virus haemagglutinating antigen in BHK-21 suspension cell cultures. Applied Microbiology 18:433–437
    [Google Scholar]
  5. Fahey J. L., Terry E. W. 1967; Ion exchange chromatography and gel filtration. In Handbook of experimental immunology pp 19–43 Edited by Weir D. M. Oxford: Blackwell;
    [Google Scholar]
  6. Folch J., Lees M., Sloane-Stanley G. H. 1957; A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry 226:497–509
    [Google Scholar]
  7. Gorman B. 1970; Lipid inhibitors of arbovirus haemagglutination. Journal of General Virology 6:305–313
    [Google Scholar]
  8. Gotto A. M., Levy R. I., Rosenthal A. S., Birnbaumer M., Fredrikson D. S. 1968; The Structure and properties of human beta-lipoprotein and beta-apoprotein. Biochemical and Biophysical Research Communications 31:699–705
    [Google Scholar]
  9. Halonen P. E., Murphy F. A., Fields B. N., Reese D. R. 1968; Hemagglutinin of rabies and some other bullet-shaped viruses. Proceedings of the Society for Experimental Biology and Medicine 127:1037–1042
    [Google Scholar]
  10. Halonen P. E., Nikkari T., Toivanen P. 1969; Nonspecific serum inhibitors of rabies and vesicular stomatitis virus hemagglutinins. The Scandinavian Journal of Clinical and Laboratory Investigation 23:108–59
    [Google Scholar]
  11. Hatch F. T., Lees R. A. 1968; Practical methods for plasma lipoprotein analysis. In Advances in Lipid Research vol 6 pp 1–68 Edited by Paoletti R., Kritchevsky D. New York: Academic Press;
    [Google Scholar]
  12. Haukenes G., Aasen J. 1971; Rubella haemagglutination inhibitors: Their separation from antibodies. Acta pathologica et microbiologica scandinavica B 79:679–685
    [Google Scholar]
  13. Havel R. J., Eder H. A., Bragdon J. H. 1955; The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. Journal of Clinical Investigation 34:1345–1353
    [Google Scholar]
  14. Kuwert E., Wiktor T. J., Sokol F., Koprowski H. 1968; Hemagglutination by rabies virus. Journal of Virology 2:1381–1392
    [Google Scholar]
  15. Nicoli J. 1965; Les recepteurs erythrocytaires des arbovirus. Les lipids globulaires. Annales del′Institut Pasteur, Paris 108:423–441
    [Google Scholar]
  16. Porterfield J. S., Rowe C. E. 1960; Hemagglutination with arthropod-borne viruses and its inhibition by certain phospholipids. Virology II765–770
    [Google Scholar]
  17. Salminen A. 1962; Chemistry of nonspecific inhibitors of hemagglutination by arthropod-borne viruses. Virology 16:201–203
    [Google Scholar]
  18. Work T. H. 1964; Isolation and identification of arthropod-borne viruses. In Diagnostic Procedures for Viral and Rickettsial Diseases pp 312–355 Edited by Lennette E. H., Schmidt N. J. New York: American Public Health Association, Inc;
    [Google Scholar]
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