Role of Lysosomes during Infection with Shope Fibroma Virus of Primary Rabbit Kidney Tissue Culture Cells

At 30 to 60 min after infection of primary rabbit kidney cells with Shope fibroma virus, the majority of virus particles were found within dense granules identified as lysosomes, whereas by 24 h no particles could be observed. The lysosomes appeared to become more fragile as infection proceeded, cellular proteins being progressively released from 7 to 24 h. Extracted lysosomal enzymes had no effect on purified Shope fibroma virus.