1887

Abstract

SUMMARY

Some activity (1 to 10%) was recovered from partially purified leukocyte interferon which had been reduced by mercaptoethanol and allowed to oxidize in air. The recovery was complete if the reduced interferon was unfolded by guanidine hydrochloride or urea. Oxidation in the continued presence of these denaturants lead to incomplete recovery (3%). Carboxymethylation of reduced interferon permanently destroyed all activity. Sodium dodecyl sulphate did not cause any loss of interferon activity but did hinder successful re-oxidation in the presence of urea. The prime importance of at least one disulphide bond in interferon structure is indicated. Further confirmation was obtained by oxidative cleavage of disulphide bonds. The lack of effect of -chloro-mercuribenzoate suggests that free thiol groups are not important for antiviral action.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-22-1-95
1974-01-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/22/1/JV0220010095.html?itemId=/content/journal/jgv/10.1099/0022-1317-22-1-95&mimeType=html&fmt=ahah

References

  1. Anfinsen C. B., Haber E., Sela M., White F. H. 1961; The kinetics of formation of native ribonuclease during oxidation of the reduced polypeptide chain. Proceedings of the National Academy of Sciences of the United States of America 47:1309–1314
    [Google Scholar]
  2. Cantell K., Pyhälä L. 1973; Circulating interferon in rabbits after administration of human interferon by different routes. Journal of General Virology 20:97–104
    [Google Scholar]
  3. Fantes K. H., O’neill C. F. 1964; Some similarities between a viral inhibitor of plant origin and chick interferon. Nature, London 203:1048–1050
    [Google Scholar]
  4. Fantes K. H. 1970; Purification and properties of human interferon. Annals of the New York Academy of Sciences 173:118–121
    [Google Scholar]
  5. Foster J. F. 1960; Plasma albumin. In The Plasma Proteins vol I pp 179 Edited by Putnam F. W. New York: Academic Press;
    [Google Scholar]
  6. Gurd F. R. N. 1967; Carboxymethylation. Methods in Enzymology 11:532–541
    [Google Scholar]
  7. Haber E., Anfinsen C. B. 1962; Side-chain interactions governing the pairing of half-cystine residue in ribonuclease. Journal of Biological Chemistry 237:1839–1844
    [Google Scholar]
  8. Hirs C. W. 1967; Performic acid oxidation. Methods in Enzymology 11:197–199
    [Google Scholar]
  9. International symposium on standardization of interferon and interferon inducers, london 1969 Symposia Series in Immunological Standardization 14326 Basel and New York: Karger;
    [Google Scholar]
  10. Jensen E. V. 1959; Disulphide interchange. Science, New York 130:1319–1323
    [Google Scholar]
  11. Ke Y. H., Ho M. 1968; Studies on the physico-chemical inactivation of rabbit interferons. Proceedings of the Society for Experimental Biology and Medicine 129:433–438
    [Google Scholar]
  12. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with Folin-phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  13. Marshall L. W., Pitha P. M., Carter W. A. 1972; Inactivation of interferon: the effect of protonation. Virology 48:607–611
    [Google Scholar]
  14. Merigan T. C., Reed S. E., Hall T. S., Tyrrell D. A. J. 1973; Inhibition of respiratory virus infection by locally applied interferon. Lancet 1:563–567
    [Google Scholar]
  15. Merigan T. C., Winget C. A., Dixon C. B. 1965; Purification and characterization of vertebrate interferons. Journal of Molecular Biology 13:679–691
    [Google Scholar]
  16. Mogensen K. E., Cantell K. 1973; Stability of human leukocyte interferon towards heat. Acta pathologica et microbiologica scandinavica B81:382–383
    [Google Scholar]
  17. Ng M. H., Vilček J. 1972; Interferons. Advances in Protein Chemistry 26:173–242
    [Google Scholar]
  18. Smithies O. 1965; Disulphide bond cleavage and formation in proteins. Science, New York 150:1595–1598
    [Google Scholar]
  19. Strander H., Cantell K. 1966; Production of interferon by human leukocytes in vitro. Annales Medicinae Experimentalis et Biologiae Fenniae 44:265–273
    [Google Scholar]
  20. Strander H., Cantell K., Carlström G., Jakobsson P. Å. 1973; Systemic administration of potent interferon to man. Journal of the National Cancer Institute (in the press)
    [Google Scholar]
  21. Tanford C. 1968; Protein denaturation. Advances in Protein Chemistry 23:122–275
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-22-1-95
Loading
/content/journal/jgv/10.1099/0022-1317-22-1-95
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error