1887

Journal of General Virology header logo

Volume 22, Issue 1

Heterogeneity of Simian Virus 40 Populations: Virus Particles Containing One or Two Virus DNA Molecules? Free

Abstract

SUMMARY

Regardless of the input multiplicity used in propagating simian virus 40 in monkey kidney cells, virus populations essentially devoid of defective viruses still contain three types of particles: heavy virus (ρ = 1.35 g/ml), light virus (ρ = 1.325 g/ml) and ‘empty’ capsids (ρ = 1.30 g/ml). Neither light nor heavy virus fractions contain lipids. The protein moiety of the three particle forms is composed of the same polypeptides in similar proportions. DNA extracted from the light virus fraction sediments at a rate identical to that of DNA from heavy virus both in neutral and alkaline sucrose density gradients. The two DNAs hybridize with the same efficiency with the DNA from heavy virus and band together after isopycnic centrifuging in CsCl solution containing ethidium bromide, indicating structural similarity. The sedimentation rates of heavy and light virus particles and their sizes as determined by electron microscopy, are also similar. However, the protein to DNA ratio in the light virus fraction is twice that in the heavy virus fraction. Thus, either heavy virus particles contain two double-stranded, circular DNA molecules of identical molecular size, and light particles contain one, or the light virus fraction contains aggregates of heavy virus and particles devoid of DNA in equal proportion. The specific infectivity of heavy virus is about ten times larger than that of light virus, but the specific infectivity of virus DNA isolated from these virus forms is similar.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1974
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-22-1-55
1974-01-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/22/1/JV0220010055.html?itemId=/content/journal/jgv/10.1099/0022-1317-22-1-55&mimeType=html&fmt=ahah

References

  1. Aloni Y., Winocour E., Sachs L. 1968; Characterization of the simian virus 40-specific RNA in virus-yielding and transformed cells. Journal of Molecular Biology 31:415–429
     [Google Scholar]
  2. Anderer F. A., Schlumberger H. D., Koch M. A., Frank H., Eggers H. J. 1967; Structure of simian virus 40 II. Symmetry and components of the virus particle. Virology 32:511–523
     [Google Scholar]
  3. Barbanti-Brodano G., Swetly P., Koprowski H. 1970; Early events in the infection of permissive cells with simian virus 40: adsorption, penetration and uncoating. Journal of Virology 6:78–86
     [Google Scholar]
  4. Berkowitz D. M., Kakefuda T., Sporn M. B. 1969; A simple and rapid method for the isolation of enzymatically active HeLa cell nuclei. The Journal of Cell Biology 42:851–855
     [Google Scholar]
  5. Black P. H., Crawford E. M., Crawford L. V. 1964; The purification of simian virus 40. Virology 24:381–387
     [Google Scholar]
  6. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochemical Journal 62:315–323
     [Google Scholar]
  7. Carp R. I., Sauer G., Sokol F. 1969; The effect of actinomycin D on the transcription and replication of simian virus 40 deoxyribonucleic acid. Virology 37:214–226
     [Google Scholar]
  8. Crawford L. V., Black P. H. 1964; The nucleic acid of simian virus 40. Virology 24:388–392
     [Google Scholar]
  9. Estes M. K., Huang E. S., Pagano J. S. 1971; Structural polypeptides of simian virus 40. Journal of Virology 7:635–641
     [Google Scholar]
  10. Hummeler K., Tomassini N., Sokol F. 1970; Morphological aspects of the uptake of simian virus 40 by permissive cells. Journal of Virology 6:87–93
     [Google Scholar]
  11. Jensen F. C., Girardi A. J., Gilden R. V., Koprowski H. 1964; Infection of human and simian tissue cultures with Rous sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 52:53–59
     [Google Scholar]
  12. Koch M. A., Eggers H. J., Anderer F. A., Schlumberger H. D., Frank H. 1967; Structure of simian virus 40.I. Purification and physical characterization of the virus particle. Virology 32:503–510
     [Google Scholar]
  13. Lowry D. H., Rosebrough R. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
     [Google Scholar]
  14. Mayor H. D., Jamison R. M., Jordan L. E. 1963; Biophysical studies on the nature of the simian papova virus particle (vacuolating SV40 virus). Virology 19:359–366
     [Google Scholar]
  15. Ozer H. L. 1972; Synthesis and assembly of simian virus 40. I. Differential synthesis of intact virions and empty shells. Journal of Virology 9:41–51
     [Google Scholar]
  16. Radloff R., Bauer W., Vinograd J. 1967; A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 57:1514–1521
     [Google Scholar]
  17. Sebring E. D., Kelly T. J. Jun, Thoren M. M., Salzman N. P. 1971; Structure of replicating simian virus 40 deoxyribonucleic acid molecules. Journal of Virology 8:478–490
     [Google Scholar]
  18. Sokol F., Schlumberger H. D., Wiktor T. J., Koprowski H., Hummeler K. 1969; Biochemical and biophysical studies on the nuleocapsid and on the RNA of rabies virus. Virology 38:651–665
     [Google Scholar]
  19. Sokol F., Stanček D., Koprowski H. 1971; Structural proteins of rabies virus. Journal of Virology 7:241–249
     [Google Scholar]
  20. Swetly P., Barbanti-Brodano G., Knowles B., Koprowski H. 1969; Response of simian virus 40-transformed cell lines and cell hybrids to superinfection with simian virus 40 and its deoxyribonucleic acid. Journal of Virology 4:348–355
     [Google Scholar]
  21. Tan K. B., Sokol F. 1972; Structural protein of simian virus 40: phosphoproteins. Journal of Virology 10:985–994
     [Google Scholar]
  22. Trilling D., Axelrod D. 1972; Analysis of the three components of simian virus 40: pseudo, mature, and defective viruses. Virology 47:360–369
     [Google Scholar]
  23. Uchida S., Watanabe S. 1968; Tumorigenicity of the antigen-forming defective virions of simian virus 40. Virology 35:166–169
     [Google Scholar]
  24. Uchida S., Watanabe S. 1969; Transformation of mouse 3T3 cells by T antigen-forming defective SV40 virions (T particles). Virology 39:721–728
     [Google Scholar]
  25. Uchida S., Yoshiike K., Watanabe S., Furuno A. 1968; Antigen-forming defective viruses of simian virus 40. Virology 34:1–8
     [Google Scholar]
  26. Yoshiike K. 1968a; Studies on DNA from low-density particles of SV40.I. Heterogeneous defective virions produced by successive undiluted passages. Virology 34:391–401
     [Google Scholar]
  27. Yoshiike K. 1968b; Studies on DNA from low-density particles of SV40. II. Noninfectious virions associated with a large-plaque variant. Virology 34:401–409
     [Google Scholar]
  28. Zur Hausen H., Sokol F. 1969; Fate of adenovirus 12 genomes in nonpermissive cells. Journal of Virology 4:256–263
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-22-1-55
Loading
Heterogeneity of Simian Virus 40 Populations: Virus Particles Containing One or Two Virus DNA Molecules?
J. Gen. Virol. 22, 55 (1974); https://doi.org/10.1099/0022-1317-22-1-55
/content/journal/jgv/10.1099/0022-1317-22-1-55
/content/journal/jgv/10.1099/0022-1317-22-1-55
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error