1887

Abstract

SUMMARY

The structural proteins of vesicular stomatitis virus (VSV) Indiana, VSV New Jersey, Chandipura virus and Piry virus were compared by using two different SDS-polyacrylamide gel electrophoresis systems. The G and M proteins of the Indiana serotype had different electrophoretic mobilities from the corresponding proteins of VSV New Jersey, Chandipura virus and Piry virus in a continuous-SDS (CONT-SDS) gel system. The three major proteins of VSV Indiana G, N and M, were distinctly different from their counterpart proteins in the other three viruses when a high-resolution discontinuous-SDS (DISC-SDS) system was used. Chandipura and Piry virus glycoproteins migrated differently when co-electrophoresed in the CONT-SDS system, whereas both their N and M proteins were different in the DISC-SDS method. During infection both Chandipura and Piry viruses produced defective T particles which contained the same four structural proteins as virus particles.

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1974-01-01
2024-04-16
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References

  1. Atkinson P. H., Summers D. F. 1971; Purification and properties of HeLa cell plasma membranes. Journal of Biological Chemistry 246:5162–5175
    [Google Scholar]
  2. Bablanian R., Eggers H. J., Tamm I. 1965; Studies on the mechanism of poliovirus-induced cell damage. I. The relation between polio-induced metabolic and morphological alteration in cultured cells. Virology 26:100–113
    [Google Scholar]
  3. Banker G. A., Cotman C. W. 1972; Measurement of free electrophoretic mobility and retardation coefficient of protein-sodium dodecyl sulfate complexes by gel electrophoresis. Journal of Biological Chemistry 247:5856–5861
    [Google Scholar]
  4. Barzilai R., Lazarus L. H., Goldblum N. 1972; Viscosity-density gradient purification of foot-and-mouth disease virus. Archiv für die gesamte Virusforschung 36:141–146
    [Google Scholar]
  5. Bishop D. H. L., Roy P. 1972; Dissociation of vesicular stomatitis virus and relation of the virion proteins to the viral transcriptase. Journal of Virology 10:234–243
    [Google Scholar]
  6. Burge B. W., Huang A. S. 1970; Comparison of membrane protein glycopeptides of sindbis virus and vesicular stomatitis virus. Journal of Virology 6:176–182
    [Google Scholar]
  7. Cartwright B., Brown F. 1972; Serological relationships between different strains of vesicular stomatitis virus. Journal of General Virology 16:391–398
    [Google Scholar]
  8. Cartwright B., Smale C. J., Brown F. 1970; Structural and biological relations in vesicular stomatitis virus. In The Biology of Large RNA Viruses pp. 115–132 Edited by Barry R. D., Mahy B. W. J. London and New York: Academic Press;
    [Google Scholar]
  9. Cartwright B., Talbot P., Brown F. 1970; The proteins of biologically active sub-units of vesicular stomatitis virus. Journal of General Virology 7:267–272
    [Google Scholar]
  10. Crick J., Cartwright B., Brown F. 1966; Interfering component of vesicular stomatitis virus. Nature, London 211:1204–1205
    [Google Scholar]
  11. Hackett A. J. 1964; A possible morphological basis for the auto interference phenomenon in vesicular stomatitis virus. Virology 24:51–59
    [Google Scholar]
  12. Kang C. Y., Prevec L. 1969; Proteins of vesicular stomatitis virus. I. Polyacrylamide gel analysis of viral antigens. Journal of Virology 3:404–413
    [Google Scholar]
  13. Kelley J. M., Emerson S. U., Wagner R. R. 1972; The glycoprotein of vesicular stomatitis virus is the antigen that gives rise to and reacts with neutralizing antibody. Journal of Virology 10:1231–1235
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  15. Maizel J. V. Jun 1971; Polyacrylamide gel electrophoresis of viral proteins. In Methods in Virology vol. V, pp. 179–246 Edited by Maramorosch K., Koprowski H. London and New York: Academic Press;
    [Google Scholar]
  16. Mudd J. A., Summers D. F. 1970; Protein synthesis in vesicular stomatitis virus-infected HeLa cells. Virology 42:328–340
    [Google Scholar]
  17. Murphy F. A., Shope R. E. 1971 Proceedings of the Second International Congress for Virology, Budapest. Workshop on Bridging groups of viruses pp 261
    [Google Scholar]
  18. Nakano J. H., Gelfand H. M., Cole J. T. 1966; Antigenic segregation of type 3 poliovirus isolates related and unrelated to Sabin’s vaccine strain with the use of modified Wecker and McBride techniques. American Journal of Epidemiology 83:130–145
    [Google Scholar]
  19. Obijeski J. F., Palmer E. L., Gafford L. G., Randall C. C. 1973; Polyacrylamide gel electrophoresis of fowlpox and vaccinia virus proteins. Virology 51:512–516
    [Google Scholar]
  20. Simons K., Kääriäinen L. 1970; Characterization of the Semliki forest virus core and envelope protein. Biochemical Biophysical Research Communication 38:981–988
    [Google Scholar]
  21. Turner J. C. 1968; Triton X-100 scintillant for carbon-14 labeled materials. International Journal of Applied Radiation and Isotopes 19:557–563
    [Google Scholar]
  22. Wagner R. R., Prevec L., Brown F., Summers D. F., Sokol F., Macleod R. 1972; Classification of rhabdovirus proteins: a proposal. Journal of Virology 10:1228–1230
    [Google Scholar]
  23. Wagner R. R., Schnaitman T. A., Synder R. M. 1969; Structural proteins of vesicular stomatitis viruses. Journal of Virology 3:395–403
    [Google Scholar]
  24. Wagner R. R., Snyder R. M., Yamazaki S. 1970; Proteins of vesicular stomatitis virus: kinetics and cellular sites of synthesis. Journal of Virology 5:548–558
    [Google Scholar]
  25. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecylsulfate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  26. Wunner W. H., Pringle C. R. 1972; Comparison of structural polypeptides from vesicular stomatitis virus (Indiana and New Jersey Serotypes) and Cocal virus. Journal of General Virology 16:1–10
    [Google Scholar]
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