1887

Abstract

SUMMARY

The proteins of the virus particles, empty capsids, and 12S subunits of foot-and-mouth disease virus were compared by polyacrylamide gel electrophoresis in neutral phosphate buffer with SDS and in 1 -propionic acid with urea. In the first system the mol. wt. of the observed components were estimated and in the second system proteins with similar mol. wt. were resolved by their different charges. The proteins were either denatured by urea, reduced by mercaptoethanol, or oxidized by performic acid. They were dissociated completely only by reduction or oxidation. By denaturation with 8 -urea, dimers with mol. wt. of about 58000 were observed. In propionic acid-urea electrophoresis, each of the three virus types A Spain, O Kaufbeuren and C Upper Bavaria showed three main proteins (VP 1, VP 2, VP 3) and a fourth fast-migrating protein, VP. The rates of migration of VP 1 and VP 3 were approx. the same in all types, whereas VP 2 showed differences between types. The component VP 4 was not present in the empty capsids or in the 12 S subunits. On elution of the proteins from the gels used in propionic acid-urea electrophoresis followed by re-electrophoresis in both systems, it was found that the relative electrophoretic migration rates of VP 1 and VP 3 were opposed in the two systems. The oxidized proteins VP 1, VP 2, and VP 3 had similar mol. wt. of about 26000 to 28000 but varied in charge. Of the native virus, only one protein was split by trypsin, leaving two detectable fragments of 9500 and 18500 daltons.

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1974-01-01
2022-01-18
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References

  1. Burroughs J. N., Rowlands D. J., Sangar D. V., Talbot P., Brown F. 1971; Further evidence for multiple proteins in the foot-and-mouth disease virus particle. Journal of General Virology 13:73–84
    [Google Scholar]
  2. Charlier G., Strobbe R., Debequen J., Leunen J. 1971; Vergelijkende studie der polypeptiden von de Mond en Klauwzeervirustypen O1, A5 en CL voor en na behandeling met trypsine. Vlaams Diergenees-kundig Tijdschrift 40:461–468
    [Google Scholar]
  3. Choules C. L., Zimm B. H. 1965; An acrylamide gel soluble in scintillation fluid: its application to electrophoresis at neutral and low pH. Analytical Biochemistry 13:336–344
    [Google Scholar]
  4. Dunker A. K., Rueckert R. R. 1969; Observations on molecular weight determinations on polyacrylamide gel. Journal of Biological Chemistry 244:5074–5080
    [Google Scholar]
  5. Dunker A. K., Rueckert R. R. 1971; Fragments generated by pH dissociation of ME-virus and their relation to the structure of the virion. Journal of Molecular Biology 58:217–235
    [Google Scholar]
  6. Liebermann H., Schulze P. 1971; Struktur des Maul-und Klauenseuche-Virus. Archiv fur experimented Veterinärmedizin 25:171–183
    [Google Scholar]
  7. Maizel J. V. 1966; Acrylamide-gel electropherogram by mechanical fractionation: Radioactive Adenovirus proteins. Science, New York 151:988–990
    [Google Scholar]
  8. Newman J. F. E., Rowlands D. J., Brown F. 1973; A physico-chemical sub-grouping of the mammalian picornaviruses. Journal of General Virology 18:171–180
    [Google Scholar]
  9. Rudloff V., Braunitzer G. 1961; Über Hämoglobine. VI. Ein Verfahren zur präparativen Gewinnung natürlicher Peptide. Hoppe-Seylers Zeitschrift für physiologische Chemie 323:129–144
    [Google Scholar]
  10. Shapiro A. L., Viñuela E., Maizel J. V. 1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS–Polyacrylamide gels. Biochemical and Biophysical Research Communications 28:815–820
    [Google Scholar]
  11. Strohmaier K., Schwoebel W., Hindennach I. 1967; Zur Proteinstruktur des Virus der Maulund Klauenseuche. Zeitschrift für Naturforschung 22b:1362–1364
    [Google Scholar]
  12. Strohmaier K. 1971 In Foot-and-Mouth Disease Virus. Session 15 Proceedings of the 2nd International Congress for Virology Edited by Melnick J. L. Basel: Karger;
    [Google Scholar]
  13. Talbot P., Brown F. 1972; A model for foot-and-mouth disease virus. Journal of General Virology 15:163–170
    [Google Scholar]
  14. Vande Woude G. F., Bachrach H. L. 1971; Number and molecular weights of foot-and-mouth disease virus capsid proteins and the effects of maleylation. Journal of Virology 7:250–259
    [Google Scholar]
  15. Vande Woude G. F., Swaney J. B., Bachrach H. L. 1972; Chemical and physical properties of foot-and-mouth disease virus: A comparison with Maus Elberfeld Virus. Biochemical and Biophysical Research Communications 48:1222
    [Google Scholar]
  16. Wild T. F., Burroughs J. N., Brown F. 1969; Surface structure of foot-and-mouth disease virus. Journal of General Virology 4:313–320
    [Google Scholar]
  17. Wildy P. 1971 In Classification and Nomenclature of Viruses. Monographs in Virology Karger: Basel;
    [Google Scholar]
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