1887

Abstract

SUMMARY

Some properties of two temperature-sensitive mutants of tobacco rattle virus are described. Experiments with mixtures of long and short particles from the mutants and the wild-type (CAM) strain showed that both mutations are in the RNA of the long particle. The mutants could not be distinguished from each other or from the wild-type virus by heat inactivation, serology, particle length or symptoms produced in a range of host plants under normal glasshouse conditions. One of the mutants was temperature-sensitive in local lesion formation in , and in production of infectious virus and RNA in , whereas the other appeared to be temperature-sensitive only in . Some possible reasons for this behaviour were investigated.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-21-3-499
1973-12-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/21/3/JV0210030499.html?itemId=/content/journal/jgv/10.1099/0022-1317-21-3-499&mimeType=html&fmt=ahah

References

  1. Bancroft J. B., Rees M. W., Dawson J. R. O., Mclean G. D., Short M. N. 1972; Some properties of a temperature-sensitive mutant of cowpea chlorotic mottle virus. Journal of General Virology 16:69–81
    [Google Scholar]
  2. Barnett O. W., Murant A. F. 1970; Host range, properties and purification of raspberry bushy dwarf virus. Annals of Applied Biology 65:435–449
    [Google Scholar]
  3. Cooper J. I., Mayo M. A. 1972; Some properties of the particles of three tobravirus isolates. Journal of General Virology 16:285–297
    [Google Scholar]
  4. Harrison B. D. 1970; Tobacco rattle virus. C.M.I/A.A.B. Descriptions of Plant Viruses no. 12 4 pp
    [Google Scholar]
  5. Jockusch H. 1968; Two mutants of tobacco mosaic virus temperature sensitive in two different functions. Virology 35:94–101
    [Google Scholar]
  6. Loening U. E., Ingle J. 1967; Diversity of RNA components in green plant tissues. Nature, London 215:363–367
    [Google Scholar]
  7. Perrier A. 1971 In Plant Photosynthetic Production. Manual of Methods chap. 17 pp 632–671 Edited by Šesták Z., Catský J., Jarvis P. G. The Hague: Dr. W. Junk, N.V;
    [Google Scholar]
  8. Robinson D. J. 1973; Inactivation and mutagenesis of tobacco rattle virus by nitrous acid. Journal of General Virology 18:215–222
    [Google Scholar]
  9. Sänger H. L. 1968; Characteristics of tobacco rattle virus: evidence that its two particles are functionally defective and mutually complementing. Molecular and General Genetics 101:346–367
    [Google Scholar]
  10. Wittmann H. G. 1964; Proteinanalysen von chemisch induzierten Mutanten des Tabakmosaikvirus. Zeitschrift für Vererbungslehre 95:333–344
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-21-3-499
Loading
/content/journal/jgv/10.1099/0022-1317-21-3-499
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error