Some properties of two temperature-sensitive mutants of tobacco rattle virus are described. Experiments with mixtures of long and short particles from the mutants and the wild-type (CAM) strain showed that both mutations are in the RNA of the long particle. The mutants could not be distinguished from each other or from the wild-type virus by heat inactivation, serology, particle length or symptoms produced in a range of host plants under normal glasshouse conditions. One of the mutants was temperature-sensitive in local lesion formation in , and in production of infectious virus and RNA in , whereas the other appeared to be temperature-sensitive only in . Some possible reasons for this behaviour were investigated.


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