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By use of [125I]-p-iodophenylisothiocyanate in a modified Edman procedure, N-terminal aspartate, glycine and serine were identified and approximately assayed in the capsid protein of purified poliovirus, strain Mahoney. Two separate preparations of virus gave similar results. These three N-terminal amino acids were recovered in amounts consistent with about 60 molecules of each per virus particle, or 1 mol of each per mol of virus protein.
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