1887

Abstract

SUMMARY

Elongated protrusions (diam. 150 to 250 nm) extending from the cell membranes, have been observed by scanning electron microscopy and in thin sections of calf kidney cells 10 h after infection with high multiplicities of parainfluenza virus 3 (PIV-3).

Unfixed, flattened PIV-3 particles, were pleomorphic with diam. ranging from 100 to 500 nm. Following early fixation of fresh virus harvests, the particles appeared spherical in the scanning and transmission electron microscopes, and had a mean diam. of 135 nm. These virus particles had not been flattened on the support film as demonstrated by goniometer analysis.

Spontaneous segmentation of unfixed PIV-3 envelopes was observed. Phospholipase C digestion of the virus resulted in a similar segmentation, producing round envelope aggregates with extending peplomers.

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/content/journal/jgv/10.1099/0022-1317-21-2-359
1973-11-01
2021-10-20
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References

  1. Allison A. C. 1971; The role of membranes in the replication of animal viruses. In International Review of Experimental Pathology vol 10 pp 181–241 Edited by Richter G. W., Epstein M. A. New York: Academic Press;
    [Google Scholar]
  2. Apostolov K., Almeida J. D. 1972; Interaction of Sendai (HVJ) virus with human erythrocytes: a morphological study of haemolysis cell fusion. Journal of General Virology 15:227–234
    [Google Scholar]
  3. Blair C. D., Robinson W. S. 1970; Replication of Sendai virus. II. Steps in virus assembly. Journal of Virology 5:639–650
    [Google Scholar]
  4. Compans R. W., Choppin P. W. 1971; The structure and assembly of influenza and parainfluenza viruses. In Comparative Virology pp 407–432 Edited by Maramorosch K., Kurstak E. New York: Academic Press;
    [Google Scholar]
  5. Compans R. W., Holmes K. V., Dales S., Choppin P. W. 1964; An electron microscopic study of moderate and virulent virus-cell interactions of the parainfluenza virus SV5. Virology 30:411–426
    [Google Scholar]
  6. Georges J. C., Guedenet J. C. 1971; Etude ultrastructurale, par l’inclusion in situ et l’hémadsorption, de cultures cellulaires infectées par myxovirus parainfluenzae 2. Annales de l’institut Pasteur 121:241–246
    [Google Scholar]
  7. Hermodsson S., Westman J. 1962; Ultrastructure of parainfluenza 3 virus. Journal of Ultrastructure Research 6:499–510
    [Google Scholar]
  8. Höglund S., Blomkvist G. 1972; Some remarks on goniometer analysis of particulate objects. Journal of Ultrastructure Research 38:199
    [Google Scholar]
  9. Höglund S., Blomkvist G., Kallberg B., Söderström T. 1973; Scanning- and transmission electron-microscopic analysis of the origin of parainfluenzavirus envelope. Journal of Ultrastructure Research 42:411
    [Google Scholar]
  10. Howe C., Morgan C., Devaux ST., Cyr K., Hsu C., Rose H. M. 1967; Morphogenesis of Type 2 Parainfluenza Virus examined by light and electron microscopy. Journal of Virology 1:215–237
    [Google Scholar]
  11. Kahn D. E., Kita J., Gillespie J. H. 1969; An electron microscopic comparison of three strains of bovine parainfluenza-3 virus. Cornell Veterinarian LIX:431–438
    [Google Scholar]
  12. Kingsbury D. K. 1972; Paramyxovirus replication. Current Topics in Microbiology and Immunology 59:1–33
    [Google Scholar]
  13. Mclean A. M., Doane F. W. 1971; The morphogenesis and cytopathology of bovine parainfluenza type 3 virus. Journal of General Virology 12:271–279
    [Google Scholar]
  14. Reed S., Boyde A. 1972; Organ cultures of respiratory epithelium infected with rhinovirus or parainfluenza virus studied in a scanning electron microscope. Infection and Immunity 6:68–76
    [Google Scholar]
  15. Reginster M., Calber-Bacq C. M. 1968; Further observations on the effects of caseinase C on the envelope of influenza and Newcastle disease viruses. Journal of Ultrastructure Research 23:144–152
    [Google Scholar]
  16. Sabatini D. D., Bensch K., Barnett R. J. 1963; Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. Journal of Cell Biology 17:19–58
    [Google Scholar]
  17. Yunis E. J., Donelli W. H. 1969; The ultrastructure of replicating Newcastle disease virus in the chick embryo chorioallantoic membrane. Virology 39:352–357
    [Google Scholar]
  18. Williams R. C., Fisher H. W. 1970; Electron microscopy of tobacco mosaic virus under conditions of minimal beam exposure. Journal of Molecular Biology 52:121–123
    [Google Scholar]
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