KB cells infected with rhinovirus type 14 (RV14) in the presence of actinomycin D synthesized two major species of virus RNA. One species had properties similar to infectious RNA extracted from virus particles. The other major species of virus RNA exhibited properties that were descriptive of replicative intermediate (RI) structures. Pulse-labelling experiments with [H]-uridine suggested that the RI structures were involved in synthesis of virus RNA. Two RNA species were isolated in minor amounts from infected cells. One species had properties descriptive of a replicative form of RNA. The second minor species was single-stranded, approximately one-half the size of the virus genome and could also be isolated from purified virus.


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