1887

Abstract

SUMMARY

The growth of alastrim virus in chick embryo cells was studied under one-step conditions at various temperatures. Limited growth was found at 37 °C but there was none at 38 °C or above. Temperature-shift experiments suggested that at 38 °C only late events were temperature-sensitive. Virus DNA synthesis, induction of early enzymes and the production of early antigens were all substantially normal at 38 °C. In a study of late events at 38 °C, particle formation was found to be almost completely inhibited. Although a few immature particles were seen by electron microscopy of thin sections, cytoplasmic DNA labelled with [H]-thymidine did not become resistant to DNase and particles containing DNA were not seen after centrifuging on sucrose density gradients. There was no late rise in DNA-dependent RNA polymerase activity. Late antigen production at 38 °C appeared normal in agar gel diffusion studies both in the time of appearance and in the number of lines present, but the LS-antigen complex was slightly reduced in amount. Production of haemagglutinin was completely suppressed at 38 °C. It is concluded that the major effect of temperature on the growth of alastrim virus is to inhibit a very early stage in particle formation.

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1973-11-01
2022-01-23
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References

  1. Appleyard G., Zwartouw H. T. 1965; The effect of ρ-fluorophenylalaninc on the replication of rabbitpox virus and its nucleic acids. Journal of General Microbiology 38:429–436
    [Google Scholar]
  2. Bedson H. S. 1964; The ceiling temperatures of pox viruses. M. D. Thesis University of London:
    [Google Scholar]
  3. Bedson H. S., Cruickshank J. G. 1969; The relation of the DNA polymerase to an early temperature-sensitive event in the replication of variola virus. Journal of General Virology 4:413–422
    [Google Scholar]
  4. Bedson H. S., Dumbell K. R. 1961; The effect of temperature on the growth of pox viruses in the chick embryo. Journal of Hygiene, Cambridge 59:457–469
    [Google Scholar]
  5. Crowle A. J. 1958; A simplified micro double-diffusion agar precipitin technique. Journal of Laboratory and Clinical Medicine 52:784–787
    [Google Scholar]
  6. Cruickshank J. G., Bedson H. S. 1968; Temperature-sensitive events in the growth of variola virus in HeLa cells. Journal of General Virology 3:255–266
    [Google Scholar]
  7. Dales S. 1963; The uptake and development of vaccinia virus in strain L cells followed with labelled viral deoxyribonucleic acid. Journal of Cell Biology 18:51–72
    [Google Scholar]
  8. Dumbell K. R., Bedson H. S. 1964; The use of ceiling temperature and reactivation in the isolation of pox virus hybrids. Journal of Hygiene, Cambridge 62:133–140
    [Google Scholar]
  9. Fenner F., Burnet F. M. 1957; A short description of the poxvirus group (vaccinia and related viruses). Virology 4:305–314
    [Google Scholar]
  10. Grimley P. M., Rosenblum E. N., Mims S. J., Moss B. 1970; Interruption by rifampicin of an early stage in vaccinia virus morphogenesis: accumulation of membranes which are precursors of virus envelopes. Journal of Virology 6:519–533
    [Google Scholar]
  11. Gurvich E. B., Marennikova S. S. 1964; Laboratory diagnosis of smallpox and similar viral diseases by means of tissue culture methods III. Additional modes of differentiating viruses of the pox group in tissue culture. Acta virologica 8:435–442
    [Google Scholar]
  12. Ichihashi Y., Matsumoto S., Dales S. 1971; Biogenesis of poxviruses: role of A-type inclusions and host cell membranes in virus dissemination. Virology 46:507–532
    [Google Scholar]
  13. Joklik W. K. 1966; The poxviruses. Bacteriological Reviews 30:33–66
    [Google Scholar]
  14. Joklik W. K. 1968; The poxviruses. Annual Review of Microbiology 22:359–390
    [Google Scholar]
  15. Joklik W. K., Becker Y. 1964; The replication and coating of vaccinia DNA. Journal of Molecular Biology 10:452–474
    [Google Scholar]
  16. Jungwirth C., Launer J. 1968; Effect of poxvirus infection on host cell DNA synthesis. Journal of Virology 2:401–408
    [Google Scholar]
  17. Kates J., Dahl R., Mielke M. 1968; Synthesis and intracellular localization of vaccinia virus deoxyribonucleic acid-dependent ribonucleic acid polymerase. Journal of Virology 2:894–900
    [Google Scholar]
  18. Katz E., Moss B. 1970; Formation of a vaccinia virus structural polypeptide from a higher molecular weight precursor: inhibition by rifampicin. Proceedings of the National Academy of Sciences of the United States of America 66:677–684
    [Google Scholar]
  19. Klemperer H. G., Haynes G. R., Shedden W. I. H., Watson D. H. 1967; A virus-specific thymidine kinase in BHK21 cells infected with herpes simplex virus. Virology 31:120–128
    [Google Scholar]
  20. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 93:265–275
    [Google Scholar]
  21. McAuslan B. R. 1969; Rifampicin inhibition of vaccinia replication. Biochemical and Biophysical Research Communications 37:289–295
    [Google Scholar]
  22. McAuslan B. R., Herde P., Pett D., Ross J. 1965; Nucleases of virus-infected animal cells. Biochemical and Biophysical Research Communications 20:586–591
    [Google Scholar]
  23. McAuslan B. R., Kates J. R. 1967; Poxvirus-induced acid deoxyribonuclease: regulation of synthesis; control of activity in vivo; purification and properties of the enzyme. Virology 33:709–716
    [Google Scholar]
  24. McCarthy K., Downie A. W. 1953; The serum antibody response to alastrim. Lancet 1:257–260
    [Google Scholar]
  25. Moss B., Rosenblum E. N., Katz E., Grimley P. M. 1969; Rifampicin: a specific inhibitor of vaccinia virus assembly. Nature, London 224:1280–1284
    [Google Scholar]
  26. Oda K., Joklik W. K. 1967; Hybridization and sedimentation studies on ‘early’ and ‘late’ vaccinia messenger RNA. Journal of Molecular Biology 27:395–419
    [Google Scholar]
  27. Pennington T. H., Follett E. A. C., Szilagyi J. F. 1970; Events in vaccinia virus-infected cells following the reversal of the antiviral action of rifampicin. Journal of General Virology 9:225–237
    [Google Scholar]
  28. Pitkanen A., McAuslan B., Hedgpeth J., Woodson B. 1968; Induction of poxvirus ribonucleic acid polymerases. Journal of Virology 2:1363–1367
    [Google Scholar]
  29. Pogo B. G. T., Dales S. 1969; Two deoxyribonuclease activities within purified vaccinia virus. Proceedings of the National Academy of Sciences of the United States of America 63:820–827
    [Google Scholar]
  30. Regan E. J., Chu E. H. Y. 1966; A convenient method for assay of DNA synthesis in synchronised human cell cultures. Journal of Cell Biology 28:139–143
    [Google Scholar]
  31. Sarov I., Joklik W. K. 1972; Studies on the nature and location of the capsid polypeptides of vaccinia virions. Virology 50:579–592
    [Google Scholar]
  32. Shedlovsky T., Smadel J. E. 1942; The LS-antigen of vaccinia. II. Isolation of a single substance containing both L- and S-activity. Journal of Experimental Medicine 75:165–178
    [Google Scholar]
  33. Smith K. O., Sharp D. G. 1960; Interaction of virus with cells in tissue cultures. I. Adsorption on and growth of vaccinia virus in L cells. Virology 11:519–532
    [Google Scholar]
  34. Watson D. H. 1962; Particle counts on herpes virus in phosphotungstate negatively stained preparations. In Electron Microscopy vol 2 (paper X4) Edited Breeze. S. S. New York: Academic Press Inc;
    [Google Scholar]
  35. Watson D. H., Shedden W. I. H., Elliot A., Tetsuka T., Wildy P., Bourgaux-Ramoisy D., Gold E. 1966; Virus specific antigens in mammalian cells infected with herpes simplex virus. Immunology 11:399–408
    [Google Scholar]
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