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Abstract

SUMMARY

We have isolated temperature-sensitive mutants of SV 40 in the lytic system by mutating the virus with nitrous acid or hydroxylamine, and characterized 11 of them. Nine mutants were grouped into two complementation classes in the productive infection of CV-1 cells. The first group contains a single mutant, which was defective in synthesizing virus DNA at the restrictive temperature, but induced the synthesis of cellular DNA. The mutants of the second class produced normal amounts of virus DNA and V-antigen at the restrictive temperature. A naturally-occurring large plaque strain belonged to this group. Both groups retained the transforming ability at the restrictive temperature. Two mutants showed no complementation with either group. One of them had the characters of the first group. The other mutant did not show temperature-sensitivity when the growth was initiated by infection with its DNA. This mutant did not transform 3T3 cells even at the permissive temperature. Rabbit kidney cells, however, were transformed by this mutant effectively at the permissive temperature but less at the restrictive.

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/content/journal/jgv/10.1099/0022-1317-21-2-227
1973-11-01
2025-04-28
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References

  1. Benjamin T. L. 1970; Host range mutants of polyoma virus. Proceedings of the National Academy of Sciences of the United States of America 67:391–399
    [Google Scholar]
  2. Di Mayorca G., Callender J., Marin G., Giordano R. 1969; Temperature-sensitive mutants of polyoma virus. Virology 38:126–133
    [Google Scholar]
  3. Dulbecco R., Eckhart W. 1970; Temperature-dependent properties of cells transformed by a thermosensitive mutant of polyoma virus. Proceedings of the National Academy of Sciences of the United States of America 67:1775–1781
    [Google Scholar]
  4. Dulbecco R., Johnson J. 1970; Interferon-sensitivity of the enhanced incorporation of thymidine into cellular DNA induced by polyoma virus. Virology 42:368–373
    [Google Scholar]
  5. Eckhart W. 1969; Complementation and transformation by temperature-sensitive mutants of polyoma virus. Virology 38:120–125
    [Google Scholar]
  6. Freeze E., Bautz E., Freeze E. B. 1961; The chemical and mutagenic specificity of hydroxylamine. Proceedings of the National Academy of Sciences of the United States of America 47:845–855
    [Google Scholar]
  7. Fried M. 1965a; Isolation of temperature-sensitive mutants of polyoma virus. Virology 25:669–671
    [Google Scholar]
  8. Fried M. 1965b; Cell-transforming ability of a temperature-sensitive mutant of polyoma virus. Proceedings of the National Academy of Sciences of the United States of America 53:486–491
    [Google Scholar]
  9. Gelb L. D., Kohne D. E., Martin M. A. 1971; Quantitation of simian virus 40 sequences in African green monkey, mouse and virus-transformed cell genomes. Journal of Molecular Biology 57:129–145
    [Google Scholar]
  10. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  11. Kimura G., Dulbecco R. 1972; Isolation and characterization of temperature-sensitive mutants of simian virus 40. Virology 49:394–403
    [Google Scholar]
  12. McCutchen J. H., Pagano J. S. 1968; Enhancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyldextran. Journal of National Cancer Institute 41:351–357
    [Google Scholar]
  13. Robb J. A., Martin R. G. 1972; Genetic analysis of simian virus 40. III. Characterization of a temperature-sensitive mutant blocked at an early stage of productive infection in monkey cells. Journal of Virology 9:956–968
    [Google Scholar]
  14. Robb J. A., Smith H. S., Scher C. D. 1972a; Genetic analysis of simian virus 40. IV. Inhibited transformation of Balb/3T3 cells by a temperature-sensitive early mutant. Journal of Virology 9:969–972
    [Google Scholar]
  15. Robb J. A., Tegtmeyer P., Martin R. J., Kit S. 1972b; Proposal for a uniform nomenclature for simian virus 40 mutants. Journal of Virology 9:562–563
    [Google Scholar]
  16. Tegtmeyer P. 1972; Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon. Journal of Virology 10:591–598
    [Google Scholar]
  17. Tegtmeyer P., Ozer H. L. 1971; Temperature-sensitive mutants of simian virus 40: Infection of permissive cells. Journal of Virology 8:516–524
    [Google Scholar]
  18. Todaro G. J. 1969; Transformation assay using cell line 3T3. In Fundamental Techniques in Virology pp 220–228 Edited by Habel K., Salzman. N. P. New York and London: Academic Press;
    [Google Scholar]
  19. Uchida S., Yoshiike K., Watanabe S., Furuno A. 1968; Antigen-forming defective viruses of simian virus 40. Virology 34:1–8
    [Google Scholar]
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