1887

Abstract

Summary

Poliovirus particles are associated with the RNA replication complexes and can be identified by velocity sedimentation and electron microscopy. After pulse-labelling with [H]-uridine beginning 3·5 h after infection, these virus particles have a radioactivity to infectivity ratio three- to eightfold higher than virus particles from other cell fractions. A specific association between the RNA replication complexes, poliovirus particles and smooth cytoplasmic membranes is shown by isopycnic sedimentation and partial resistance to enzyme digestion. These results strongly suggest that virus RNA replication and particle formation are coupled processes which occur in association with smooth cytoplasmic membranes.

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/content/journal/jgv/10.1099/0022-1317-21-1-99
1973-10-01
2019-11-17
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-21-1-99
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