Conditions suitable for reconstitution of the CAM strain of TRV have been defined, and various steps of rod morphogenesis have been studied.

The CAM strain of TRV was found to reconstitute best at pH 4·7 in 0·5 -phosphate buffer at low temperature, unlike the C isolate of TRV which was shown by Semancik & Reynolds (1969) to reconstitute partially at pH 8.0 in glycine buffer.

While the initiation step consisting of the binding of a RNA molecule to a 36 S double layer protein disk took place under wide pH and ionic strength ranges, elongation proved to be a much more refined process which required well-defined conditions of pH, ionic strength and temperature.


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