1887

Abstract

SUMMARY

Electrophoretic analysis of native and denatured RNA of feline leukaemia virus (FeLV) has revealed small but significant size differences between the RNAs of a number of FeLV isolates. The mol. wt. of the denatured or subunit RNA has been estimated to range from 2.2 to 2.6 × 10 in the different isolates, each of which belonged to one or more FeLV subgroups (A, B and C). The RNA of subgroup A virus was smaller than that of subgroup B virus, while the RNA of subgroup C virus appeared to be intermediate in size between the RNAs of A and B subgroup viruses. Size differences were, however, also found between RNAs of FeLV isolates of a single subgroup (A), indicating that no simple relationship exists between RNA size and virus subgroup.

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/content/journal/jgv/10.1099/0022-1317-21-1-39
1973-10-01
2024-04-19
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References

  1. Bishop D. H. L., Claybrook J. R., Spiegelman S. 1967; Electrophoretic separation of viral nucleic acids on polyacrylamide gels. Journal of Molecular Biology 26:373–387
    [Google Scholar]
  2. Bishop J. M., Levinson W. E., Quintrell N., Sullivan D., Fanshier L., Jackson J. 1970; The low molecular weight RNAs of Rous sarcoma virus. I. The 4 S RNA. Virology 42:182–195
    [Google Scholar]
  3. Bolognesi D. P., Graf T. 1971; Size differences among the high molecular weight RNAs of avian tumour viruses. Virology 43:214–222
    [Google Scholar]
  4. Burness A. T. H. 1970; Ribonucleic acid content of encephalomyocarditis virus. Journal of General Virology 6:373–380
    [Google Scholar]
  5. Canaani E., Duesberg P. 1972; Role of subunits of 60 to 70 S avian tumor virus ribonucleic acid in its template activity for the viral deoxyribonucleic acid polymerase. Journal of Virology 10:23–31
    [Google Scholar]
  6. Canaani E., Helm K. V. D., Duesberg P. 1973; Evidence for 30–40 S RNA as precursor of the 60–70 S RNA of Rous sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 70:401–405
    [Google Scholar]
  7. Cheung K. S., Smith R. E., Stone M. P., Joklik W. J. 1972; Comparison of immature (rapid harvest) and mature Rous sarcoma virus particles. Virology 50:851–864
    [Google Scholar]
  8. Duesberg P. H., Vogt P. K. 1970; Differences between the ribonucleic acids of transforming and non-transforming avian tumor viruses. Proceedings of the National Academy of Sciences of the United States of America 67:1673–1680
    [Google Scholar]
  9. Jarrett O., Laird H. M., Hay D. 1972; Restricted host range of a feline leukaemia virus. Nature, London 238:220–221
    [Google Scholar]
  10. Jarrett O., Laird H. M., Hay D. 1973; Determinants of the host range of feline leukaemia viruses. Journal of General Virology 20:169–175
    [Google Scholar]
  11. Jarrett O., Pitts J. D., Whalley J. M., Hay D., Clasen A. 1971; Isolation of the nucleic acid of feline leukaemia virus. Virology 43:317–320
    [Google Scholar]
  12. Lai M. M. C., Duesberg P. H. 1972; Differences between the envelope glycoproteins and glycopeptides of avian tumor viruses released from transformed and non-transformed cells. Virology 50:359–372
    [Google Scholar]
  13. Loening U. E. 1967; The fractionation of high molecular weight ribonucleic acid by polyacrylamide gel electrophoresis. Biochemical Journal 102:251–257
    [Google Scholar]
  14. Loening U. E. 1969; The determination of the molecular weights of ribonucleic acid by polyacrylamide gel electrophoresis. The effects of changes in conformation. Biochemical Journal 113:131–138
    [Google Scholar]
  15. Martin G. S., Duesberg P. H. 1972; The a subunit in the RNA of transforming avian tumor viruses. I. Occurrence in different virus strains. II. Spontaneous loss resulting in non-transforming variants. Virology 47:494–497
    [Google Scholar]
  16. Maruyama H. B., Hatanaka M., Gilden R. V. 1971; The 3′ terminal nucleosides of the high molecular weight RNA of C-type viruses. Proceedings of the National Academy of Sciences of the United States of America 68:1999–2001
    [Google Scholar]
  17. Montagnier L., Golde A., Vigier P. 1969; A possible subunit structure of Rous sarcoma virus RNA. Journal of General Virology 4:449–452
    [Google Scholar]
  18. Peacock A. C., Dingman C. W. 1968; Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry 7:668–674
    [Google Scholar]
  19. Penman S., Greenberg H., Willems M. 1969 In Fundamental Techniques in Virology ch. 6 pp 49–58 Edited by Habel K., Salzman N. P. New York and London: Academic Press;
    [Google Scholar]
  20. Sarma P. S., Log T. 1971; Viral interference in feline leukaemia-sarcoma complex. Virology 44:352–358
    [Google Scholar]
  21. Sarma P. S., Log T. 1973; Viral envelope antigens of feline leukaemia and sarcoma viruses. Proceedings of the Vth Symposium of the World Committee for Comparative Leukaemia Research, Padova 1971 (in the press)
    [Google Scholar]
  22. Watson J. D. 1971; The structure and assembly of murine leukemia virus intracellular viral RNA. Virology 45:586–597
    [Google Scholar]
  23. Whalley J. M. 1973; Electron microscopy of feline leukaemia virus RNA. Proceedings of the Vth Symposium of the World Committee for Comparative Leukaemia Research, Padova 1971 (in the press)
    [Google Scholar]
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