1887

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Volume 20, Issue 3

Genetic Complementation between Middle and Bottom Components of Two Strains of Radish Mosaic Virus Free

Abstract

SUMMARY

Kale virus (KV), turnip virus (HZ) and radish mosaic virus (RMV) are slightly related serologically to cowpea mosaic virus. Purified preparations comprize, top, middle and bottom components, of which only the last two contain nucleic acid. The nucleoprotein components of each virus were separated by centrifuging twice through a sucrose gradient in an MSE BXIV zonal rotor. Sometimes this was followed by centrifuging to equilibrium in a density gradient of rubidium bromide. Neither nucleoprotein component was infective alone, but infectivity was restored when each was mixed with the other component.

Mixtures in which the two components came from different viruses were infective only when they were made with KV and HZ. Serologically these two viruses are more closely related than either is to RMV. KV differed from HZ by two characters related to the coat protein. KV particles regularly formed aggregates of 12 particles and contained antigens that were not present in HZ. Single lesion isolates from mixtures of bottom component from one strain and middle component from the other did not form aggregates and lacked the antigens present in KV. Like cowpea mosaic virus, KV and HZ each have two coat proteins, and our results can be explained if each of the four virus components codes for a different coat protein. Therefore, the coat protein of the virus is coded by the nucleic acid of both middle and bottom components.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1973
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1973-09-01
2024-04-16
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References

  1. Agrawal H. O. 1964; Identification of cowpea mosaic virus isolates. Mededelingen Landbouwhogeschool Wageningen 64:1–53
     [Google Scholar]
  2. Bruening G. 1969; The inheritance of top component formation in cowpea mosaic virus. Virology 37:577–584
     [Google Scholar]
  3. Bruening G., Agrawal H. O. 1967; Infectivity of a mixture of cowpea mosaic virus ribonucleoprotein components. Virology 32:306–320
     [Google Scholar]
  4. Campbell R. N. 1964; Radish mosaic virus, a crucifer virus serologically related to strains of bean pod mottle and to squash mosaic virus. Phytopathology 54:1418–1424
     [Google Scholar]
  5. Campbell R. N., Colt W. M. 1967; Transmission of radish mosaic virus. Phytopathology 57:502–509
     [Google Scholar]
  6. De Jager C. P., Van Kammen A. 1970; The relationship between the components of cowpea mosaic virus. III. Location of genetic information for two biological functions in the middle component of CPMV. Virology 41:281–287
     [Google Scholar]
  7. Geelen J. L. M. C., Van Kammen A., Verduin B. J. M. 1972; Structure of the capsids of cowpea mosaic virus. The chemical subunit: molecular weight and number of subunits per particle. Virology 49:205–213
     [Google Scholar]
  8. Harrison B. D., Murant A. F., Mayo M. A. 1972; Two properties of raspberry ringspot virus determined by its smaller RNA. Journal of General Virology 17:137–141
     [Google Scholar]
  9. Kassanis B., Woods R. D. 1968; Aggregated forms of the satellite of tobacco necrosis virus. Journal of General Virology 2:395–398
     [Google Scholar]
  10. Lister R. M. 1966; Possible relationship of virus-specific products of tobacco rattle virus infection. Virology 28:350–353
     [Google Scholar]
  11. Markham R. 1960; A graphical method for the rapid determination of sedimentation coefficients. Biochemical Journal 77:516–519
     [Google Scholar]
  12. Markham R. 1962; The analytical ultracentrifuge as a tool for the investigation of plant viruses. Advances in Virus Research 9:241–270
     [Google Scholar]
  13. ŠTefanac Z., Mamula D. 1971; A strain of radish mosaic virus occurring in turnip in Yugoslavia. Annals of Applied Biology 69:229–234
     [Google Scholar]
  14. Van Kammen A. 1967; Purification and properties of the components of cowpea mosaic virus. Virology 31:633–642
     [Google Scholar]
  15. Van Kammen A. 1968; The relationship between the two components of cowpea mosaic virus. I. Two ribonucleoprotein particles necessary for the infectivity of CPMV. Virology 34:312–318
     [Google Scholar]
  16. Van Kammen A., Van Griensven L. J. I. D. 1970; The relationship between the components of cowpea mosaic virus. II. Further characterization of the nucleoprotein components of CPMV. Virology 41:274–280
     [Google Scholar]
  17. Van Vloten-Doting L., Dingjan-Versteegh A., Jaspers E. M. J. 1970; Three nucleoprotein components of alfalfa mosaic virus necessary for infectivity. Virology 40:419–430
     [Google Scholar]
  18. White R. F., Kassanis B., Woods R. D. 1973; Isopycnic banding of strains of radish mosaic virus in rubidium bromide solutions. Journal of General Virology 20:387–389
     [Google Scholar]
  19. Wood H. A. 1972; Genetic complementation between the two nucleoprotein components of cowpea mosaic virus. Virology 49:592–598
     [Google Scholar]
  20. Wu G., Bruening G. 1971; Two proteins from cowpea mosaic virus. Virology 46:596–612
     [Google Scholar]
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Genetic Complementation between Middle and Bottom Components of Two Strains of Radish Mosaic Virus
J. Gen. Virol. 20, 277 (1973); https://doi.org/10.1099/0022-1317-20-3-277
/content/journal/jgv/10.1099/0022-1317-20-3-277
/content/journal/jgv/10.1099/0022-1317-20-3-277
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