Thermal Inactivation of Untreated and Gamma-irradiated A2/Aichi/2/68 Influenza Virus Free

Abstract

SUMMARY

The infectivity and the haemagglutinin activity of A2/Aichi/2/68 influenza virus were unchanged during a 2-year storage in allantoic fluid at -80 °C. Over the temperature range from -20 to + 37 °C, exponential slopes could be drawn by means of the regression analysis, the velocity constants showing very low values. Conversely, at + 56 °C inactivation took place in a two-component fashion, each following first-order kinetics. Haemagglutinin and neuraminidase activities were not impaired by exposure to [Co]--rays (3 × 10 rad), which completely removed infectious particles. The specific rate constants for inactivation of haemagglutinin and neuraminidase activities of untreated and irradiated virus were overlapping when samples were stored for 2 years in the frozen state (- 80 and - 20 °C), whereas a significantly increasing rate of inactivation was recorded for -irradiated samples following storage at temperature above 4 °C. Nevertheless, the energy of activation required for thermal inactivation was very low and the entropy of activation showed negative values for both the untreated and the irradiated virus preparations.

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1973-08-01
2024-03-28
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References

  1. Black F. L. 1959; Growth and stability of measles virus. Virology 7:184–192
    [Google Scholar]
  2. Compans R. W., Klenk H. D., Caliguiri L. A., Choppin P. W. 1970; Influenza virus proteins. I. Analysis of polypeptides of the virion and identification of spike glycoproteins. Virology 42:880–889
    [Google Scholar]
  3. De Flora S., Badolati G. 1970; Studies on gamma-irradiated A2/Hong Kong influenza virus. Bollettino dell’lstituto sieroterapico Milanese 49:373–381
    [Google Scholar]
  4. Eyring H. 1935; The activated complex in chemical reactions. Journal of Chemical Physics 3:107–115
    [Google Scholar]
  5. Ginoza W. 1958; Kinetics of heat inactivation of ribonucleic acid of TMV. Nature, London 181:958–961
    [Google Scholar]
  6. Laver W. G. 1971; Separation of two polypeptide chains from the hemagglutinin subunit of influenza virus. Virology 45:275–288
    [Google Scholar]
  7. Pollard E. 1953 In The Physics of Viruses New York: Academic Press;
    [Google Scholar]
  8. Schultze I. T. 1970; The structure of influenza virus. I. The polypeptides of the virion. Virology 42:890–904
    [Google Scholar]
  9. Skehel J. J., Schild G. C. 1971; The polypeptide composition of influenza A viruses. Virology 44:396–408
    [Google Scholar]
  10. Webster R. G., Laver W. G. 1967; Preparations and properties of antibody directed specifically against the neuraminidase of influenza virus. Journal of Immunology 99:49–55
    [Google Scholar]
  11. Webster R. G. 1970; Estimation of the molecular weights of the polypeptide chains from the isolated hemagglutinin and neuraminidase subunits of influenza viruses. Virology 40:643–654
    [Google Scholar]
  12. Woese C. 1960; Thermal inactivation of animal viruses. New York Academy of Sciences 83:741–751
    [Google Scholar]
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