1887

Abstract

SUMMARY

The infectivity and the haemagglutinin activity of A2/Aichi/2/68 influenza virus were unchanged during a 2-year storage in allantoic fluid at -80 °C. Over the temperature range from -20 to + 37 °C, exponential slopes could be drawn by means of the regression analysis, the velocity constants showing very low values. Conversely, at + 56 °C inactivation took place in a two-component fashion, each following first-order kinetics. Haemagglutinin and neuraminidase activities were not impaired by exposure to [Co]--rays (3 × 10 rad), which completely removed infectious particles. The specific rate constants for inactivation of haemagglutinin and neuraminidase activities of untreated and irradiated virus were overlapping when samples were stored for 2 years in the frozen state (- 80 and - 20 °C), whereas a significantly increasing rate of inactivation was recorded for -irradiated samples following storage at temperature above 4 °C. Nevertheless, the energy of activation required for thermal inactivation was very low and the entropy of activation showed negative values for both the untreated and the irradiated virus preparations.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-20-2-261
1973-08-01
2021-09-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/20/2/JV0200020261.html?itemId=/content/journal/jgv/10.1099/0022-1317-20-2-261&mimeType=html&fmt=ahah

References

  1. Black F. L. 1959; Growth and stability of measles virus. Virology 7:184–192
    [Google Scholar]
  2. Compans R. W., Klenk H. D., Caliguiri L. A., Choppin P. W. 1970; Influenza virus proteins. I. Analysis of polypeptides of the virion and identification of spike glycoproteins. Virology 42:880–889
    [Google Scholar]
  3. De Flora S., Badolati G. 1970; Studies on gamma-irradiated A2/Hong Kong influenza virus. Bollettino dell’lstituto sieroterapico Milanese 49:373–381
    [Google Scholar]
  4. Eyring H. 1935; The activated complex in chemical reactions. Journal of Chemical Physics 3:107–115
    [Google Scholar]
  5. Ginoza W. 1958; Kinetics of heat inactivation of ribonucleic acid of TMV. Nature, London 181:958–961
    [Google Scholar]
  6. Laver W. G. 1971; Separation of two polypeptide chains from the hemagglutinin subunit of influenza virus. Virology 45:275–288
    [Google Scholar]
  7. Pollard E. 1953 In The Physics of Viruses New York: Academic Press;
    [Google Scholar]
  8. Schultze I. T. 1970; The structure of influenza virus. I. The polypeptides of the virion. Virology 42:890–904
    [Google Scholar]
  9. Skehel J. J., Schild G. C. 1971; The polypeptide composition of influenza A viruses. Virology 44:396–408
    [Google Scholar]
  10. Webster R. G., Laver W. G. 1967; Preparations and properties of antibody directed specifically against the neuraminidase of influenza virus. Journal of Immunology 99:49–55
    [Google Scholar]
  11. Webster R. G. 1970; Estimation of the molecular weights of the polypeptide chains from the isolated hemagglutinin and neuraminidase subunits of influenza viruses. Virology 40:643–654
    [Google Scholar]
  12. Woese C. 1960; Thermal inactivation of animal viruses. New York Academy of Sciences 83:741–751
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-20-2-261
Loading
/content/journal/jgv/10.1099/0022-1317-20-2-261
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error