1887

Abstract

SUMMARY

Hepatitis B (Australia) antigen was isolated from normal serum proteins by gel filtration and electrofocusing. The latter technique revealed a heterogeneity of the small spherical particles associated with hepatitis B antigen activity, there being a variance in determined isoelectric points according to the serological subtype studied. Hepatitis Bantigen isolated in this manner was aggregated by concanavalin A.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-20-2-253
1973-08-01
2024-12-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/20/2/JV0200020253.html?itemId=/content/journal/jgv/10.1099/0022-1317-20-2-253&mimeType=html&fmt=ahah

References

  1. Bond H. E. 1972; In discussion following ‘Isolation and Physiochemical Characteristics of HB Ag’ by J. Gerin. In Hepatitis and Blood Transfusion p 205 Edited by Vyas, G. N. Perkins H. A., Schmid R. New York: Greive and Stratton;
    [Google Scholar]
  2. Burrell C. J., Proudfoot E., Keen G. A., Marmion B. P. 1973; Carbohydrate in hepatitis B antigen. Nature, London (in the press)
    [Google Scholar]
  3. Dreesman G. R., Hollinger F. B., Suriano J. R., Fujioka R. S., Brunschwig J. P., Melnick J. L. 1972; Biophysical and biochemical heterogeneity of purified hepatitis B antigen. Journal of Virology 10:469–476
    [Google Scholar]
  4. Gerin J. L., Purcell R. H., Hoggan M. D., Holland P. V., Channock R. M. 1969 Journal of Virology 4:763–768
    [Google Scholar]
  5. Hung P. P., Robinson H. L., Robinson W. S. 1971; Isolation and characterization of proteins from Rous sarcoma virus. Virology 43:251–266
    [Google Scholar]
  6. Kim C. Y., Bissell D. M. 1971; Stability of the lipid and protein of hepatitis-associated (Australia) antigen. Journal of Infectious Diseases 123:470–476
    [Google Scholar]
  7. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–274
    [Google Scholar]
  8. Memorandum 1970; Tests for Australia (hepatitis-associated) antigen and antibody. Bulletin of the World Health Organization 42:957–992
    [Google Scholar]
  9. Millman I., Loeb L. A., Bayer M. E., Blumberg B. S. 1970; Australia antigen (a hepatitis-associated antigen). Purification and physical properties. Journal of Experimental Medicine 131:1190–99
    [Google Scholar]
  10. Rice R. H., Horst J. 1972; Isoelectric focusing of viruses in polyacrylamide gels. Virology 49:602–604
    [Google Scholar]
  11. Shortridge K. F., Biddle F. 1970; The proteins of adenovirus type 5. Archiv fur die gesamte Virusfor-schung 29:1–24
    [Google Scholar]
  12. Vyas G. N., Williams E. W., Klauss G. G. B., Bond H. E. 1972; Hepatitis-associated Australian antigen. Protein, peptides and amino-acid composition of purified antigen with its use in determining sensitivity of the haemagglutination test. Journal of Immunology 108:1114–1118
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-20-2-253
Loading
/content/journal/jgv/10.1099/0022-1317-20-2-253
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error