1887

Abstract

SUMMARY

A mutant of the virus of foot-and-mouth disease (O type) was studied, and shown by electrophoresis in SDS-polyacrylamide gels to contain equimolar proportions of four polypeptides with mol. wts. of 34, 20, 17 and 14 × 10. Two minor components were also present with mol. wts. of 51 and 25 × 10 and in the molar ratio of 0.07 and 0.28. Only the polypeptide of mol. wt. 34 × 10 was labelled when the intact virus was iodinated. This polypeptide was sensitive to the action of trypsin and showed leucine as the -terminal amino acid. Our own and other results are discussed in terms of virus structure.

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1973-08-01
2024-12-07
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References

  1. Asso J. 1967; Etude de quelques mutants du virus de la Fievre aphteuse. Ph.D. Thesis University of Paris;
    [Google Scholar]
  2. Bachrach H. L., Trautman R., Breese S. S. Jun 1964; Chemical and physical properties of virtually pure Foot-and-Mouth disease virus. American Journal of Veterinary Research 25:333–342
    [Google Scholar]
  3. Bray G. A. 1960; A simple effective liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analytical biochemistry 1:279–285
    [Google Scholar]
  4. Burroughs J. N., Rowlands D. J., Sangar D. V., Talbot P., Brown F. 1971; Further evidence for multiple proteins in the Foot-and-Mouth disease virus particle. Journal of General Virology 13:73–84
    [Google Scholar]
  5. Caspar D. L. D., Klug A. 1962 Cold Spring Harbor Symposia on Quantitative Biology vol 27:
    [Google Scholar]
  6. Helmkamp R. W., Goodland R. L., Bale W. F., Spar I. L., Mutschiler L. E. 1960; High Specific activity Sodination of y globulin with iodine-131 monochloride. Cancer Research 20:1495–1500
    [Google Scholar]
  7. Johnston M. O., Martin S. S. 1971; Capsid and Procapsid proteins of a bovine enterovirus. Journal of General Virology n:71–79
    [Google Scholar]
  8. Laporte J. 1968 Reunion de la Societe fran$aise de Microbiologie—Section Virologie (Strasbourg 11 Mai 1968)
    [Google Scholar]
  9. Laporte J. 1969; The structure of Foot-and-Mouth disease virus protein. Journal of General Virology 4:631–634
    [Google Scholar]
  10. Macfarlane A. S. 1958; Efficient trace-labelling of proteins with iodine. Nature, London 182:53
    [Google Scholar]
  11. Macpherson J. A., Stoker M. G. P. 1962; Polyoma transformation of Hamster cells clones-an investigation for genetic factors affecting cells competence. Virology 16:147–151
    [Google Scholar]
  12. Rueckert R. R., Dunker A. K., Stoltzfus C. M. 1969; The structure of mouse Elberfeld virus: a model. Proceedings of the National Academy of Sciences of the United States of America 62:912–919
    [Google Scholar]
  13. Shapiro A. L., Vinuela E., Maizel J. V. 1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS-Polyacrylamide gels. Biochemical and Biophysical Research Communications 28:815–820
    [Google Scholar]
  14. Summers D., Maizel J. V., Darnell J. E. 1965; Evidence for virus-specific noncapsid proteins in Poliovirus infected HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 54:505–513
    [Google Scholar]
  15. Talbot P., Brown F. 1972; A model of Foot-and-Mouth disease virus. Journal of General Virology 15:163–170
    [Google Scholar]
  16. van de Woude G. F., Bachrach H. L. 1968; Evidence for a single structural polypeptide in Foot-and-Mouth disease virus. Archiv fur die gesamte Virusforschung 23:353–361
    [Google Scholar]
  17. van de Woude G. F., Bachrach H. L. 1971; Number and Molecular weight of Foot-and-Mouth disease virus capsid proteins and the effect of Maleylation. Journal of Virology 7:250–259
    [Google Scholar]
  18. Wild F. F., Burroughs J. N., Brown F. 1969; Surface structure of Foot-and-Mouth disease virus. Journal of General Virology 4:313–319
    [Google Scholar]
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