1887

Abstract

SUMMARY

Examination of chloroplasts from chinese cabbage leaves infected with turnip yellow mosaic virus (TYMV), using freeze-fracture techniques revealed, that the small peripheral vesicles induced by infection were present in clusters near the surface of the chloroplast, often being arranged in arrays that approximate to hexagonal. The small peripheral vesicles were flask shaped with their necks pointing to the chloroplast surface. The structure of the necks, which were only about 15 to 22 nm in diam., suggested but did not prove that they were open to the cytoplasmic space.

The inner membrane of the peripheral vesicles is distinguished from other chloroplast membranes in showing no particles on either the A or the B fracture faces.

In the regions where the peripheral vesicles were clustered, the number and kind of particles seen on the chloroplast membrane faces were changed compared with healthy chloroplasts or the non-vesicle bearing areas of chloroplasts from infected cells. In particular, the B face of the outer chloroplast membrane in the vesicle-bearing regions had a reduced number of particles 10 nm in diam., but in addition to these contained large numbers of particles about 5 nm in diam.

In the chloroplasts of both healthy and infected tissue we frequently observed protuberances of the stroma corresponding to the mobile phase of chloroplasts postulated from light microscopic observations.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-20-1-37
1973-07-01
2022-08-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/20/1/JV0200010037.html?itemId=/content/journal/jgv/10.1099/0022-1317-20-1-37&mimeType=html&fmt=ahah

References

  1. Bächi T., Gerhard W., Lindenmann J., Mühlethaler K. 1969; Morphogenesis of Influenza virus A in Erlich Ascites Tumour Cells as revealed by thin sectioning and freeze-etching. Journal of Virology 4:769–776
    [Google Scholar]
  2. Branton D. 1966; Fracture faces of frozen membranes. Proceedings of the National Academy of Sciences of the United States of America 55:1048–1056
    [Google Scholar]
  3. Branton D. 1969; Membrane structure. Annual Review of Plant Physiology 20:209–238
    [Google Scholar]
  4. Branton D. 1971; Freeze etching studies of membrane structure. Philosophical Transactions of the Royal Society of London. B 261:133–138
    [Google Scholar]
  5. Brown D. T., Waite M. R. F., Pfefferkorn E. R. 1972; Morphology and morphogenesis of Sindbis virus as seen with freeze etching techniques. Journal of Virology 10:524–536
    [Google Scholar]
  6. Bruns R. R., Palade G. E. 1968; Studies on blood capillaries. II. Transport of ferritin molecules across the wall of muscle capillaries. Journal of Cell Biology 37:277–299
    [Google Scholar]
  7. Bullivant S., Ames A. 1966; A simple freeze-fracture replication method for electron microscopy. Journal of Cell Biology 29:435–447
    [Google Scholar]
  8. Bullivant S. 1969; Freeze-fracturing of biological materials. Micron 1:46–51
    [Google Scholar]
  9. Chalcroft J. P., Bullivant S. 1970; An interpretation of liver cell membrane and junction structure based on observation of freeze-fracture replicas of both sides of the fracture. Journal of Cell Biology 47:49–60
    [Google Scholar]
  10. Chalcroft J., Matthews R. E. F. 1966; Cytological changes induced by turnip yellow mosaic virus in Chinese cabbage leaves. Virology 28:555–562
    [Google Scholar]
  11. Chalcroft J., Matthews R. E. F. 1967; Role of virus strains and leaf ontogeny in the production of mosaic patterns by turnip yellow mosaic virus. Virology 33:659–673
    [Google Scholar]
  12. Laflèche D., Bové J. M. 1969; Development of double membrane vesicles in chloroplasts from turnip yellow mosaic virus infected cells. Progress in Photosynthesis Research 1:74–83
    [Google Scholar]
  13. Mcnutt N. S., Weinstein R. S. 1970; The ultrastructure of the nexus. A correlated thin-section and freeze-cleave study. Journal of Cell Biology 47:666–688
    [Google Scholar]
  14. Matthews R. E. F. 1970 In Plant Virology 778 pp New York and London: Academic Press;
    [Google Scholar]
  15. Matthews R. E. F. 1973; Induction of disease by viruses with special reference to turnip yellow mosaic virus. Annual Reviews of Phytopathology (in the press)
    [Google Scholar]
  16. Millonig G. 1961; Advantages of a phosphate buffer for OsO4 solutions in fixation. Journal of Applied Physics 32:1637
    [Google Scholar]
  17. Moor H. 1971; Recent progress in the freeze-etching technique. Philosophical Transactions of the Royal Society of London. B 261:121–131
    [Google Scholar]
  18. Nickel E., Grieshaber E. 1969; Elektronenmikroskopische Darstellung der Muskelkapillaren im Gefriertazbild. Zeitschrift für Zellforschung und mikroskopische Anatomie 95:445–461
    [Google Scholar]
  19. Revel J. P., Karnovsky M. J. 1967; Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. Journal of Cell Biology 33:C7–C12
    [Google Scholar]
  20. Roth S., Mcguire E. J., Roseman S. 1971; An assay for intercellular adhesive specificity. Journal of Cell Biology 51:525–535
    [Google Scholar]
  21. Ushiyama R., Matthews R. E. F. 1970; The significance of chloroplast abnormalities associated with infection by turnip yellow mosaic virus. Virology 42:293–303
    [Google Scholar]
  22. Wildman S. G. 1967; The organisation of grana-containing chloroplasts in relation to location of some enzymatic systems concerned with photosynthesis, protein synthesis and ribonucleic acid synthesis. In Biochemistry of Chloroplasts 2295–319 (Proceedings of a NATO Advanced Study Institute. Aberystwyth, 1965) Edited by Goodwin T. W. New York and London: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-20-1-37
Loading
/content/journal/jgv/10.1099/0022-1317-20-1-37
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error