1887

Abstract

SUMMARY

Steady-state chemostat cultures of mouse LS-cells grown in a chemically defined, protein-free medium were induced to produce interferon by a mycophage double-stranded RNA in a system potentiated by DEAE-dextran. The induction was terminated by the addition of heparin. The kinetics of interferon production in glucose-limited cultures were similar to those produced by the same induction system in batch culture. A maximum titre of approximately 2.8 log units/ml was reached 7 to 8 h postinduction. Interferon production was unaffected by changes in the cell growth rate within the range of dilution rates 0.25 to 0.35 day. Glucose-limited chemostat cultures (0.5 mg glucose/ml) yielded higher titres of interferon than cultures with excess glucose. Repeated induction of interferon was obtained in the chemostat without the development of a refractory state, although the repeated treatment was associated with inhibition of cell growth.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-20-1-29
1973-07-01
2022-08-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/20/1/JV0200010029.html?itemId=/content/journal/jgv/10.1099/0022-1317-20-1-29&mimeType=html&fmt=ahah

References

  1. Auricchio F., Martin D., Tomkins O. 1969; Control of degradation and synthesis of induced tyrosine aminotransferase studied in Hepatoma cells in culture. Nature, London 224:806–808
    [Google Scholar]
  2. Birch J. R., Pirt S. J. 1970; Improvements in a chemically defined medium for the growth of mouse cells (strain LS) in suspension. Journal of Cell Science 7:666–676
    [Google Scholar]
  3. Buck K. W., Chain E. B., Himmelweit E. 1971; Comparison of interferon induction in mice by purified Penicillium chrysogenum virus and derived double-stranded RNA. Journal of General Virology 12:131–139
    [Google Scholar]
  4. Finter N. B. 1969; Dye uptake methods for assaying viral cytopathogenicity and their application to interferon assays. Journal of General Virology 5:419–427
    [Google Scholar]
  5. Friedmann T. E., Haugen G. L. 1943; Pyruvic acid II. The determination of keto acids in blood and urine. Journal of Biological Chemistry 147:415–422
    [Google Scholar]
  6. Gresser I., Brouty-Boye D., Thomas M. T., Macieira-Coelho A. 1970; Interferon and cell division. I. Inhibition of the multiplication of mouse leukemia L 1210 cells in vitro by interferon preparations. Proceedings of the National Academy of Sciences of the United States of America 66:1052–1058
    [Google Scholar]
  7. Ho M., Tan Y. H., Armstrong J. A. 1972; Accentuation of production of human interferon by metabolic inhibitors. Proceedings of the Society for Experimental Biology and Medicine 134:259–262
    [Google Scholar]
  8. Lockart R. Z. Jun 1966; Biological properties of interferon. Criteria for acceptance of a viral inhibitor as an interferon. In The Interferons Edited by Finter N. B. Amsterdam: North Holland Publishing Co;
    [Google Scholar]
  9. Loomis W. F., Magasanik B. 1964; The relation of catabolite repression to the induction system for β-galactosidasc in Escherichia coli. Journal of Molecular Biology 8:417–426
    [Google Scholar]
  10. Mcfall E., Mandelstam J. 1963; Specific metabolic repression of induced enzymes in Escherchia coli. Nature, London 197:880–881
    [Google Scholar]
  11. Mogensen K. E., Tovey M. G., Pirt S. J., Mathison G. E. 1972; Induction of mouse interferon in a chemically defined system. Journal of General Virology 16:111–114
    [Google Scholar]
  12. Myers M. W., Friedman R. M. 1971; Potentiation of human interferon production by superinduction. Journal of National Cancer Institute 47:757–764
    [Google Scholar]
  13. Pirt S. J., Callow D. S. 1964; Continuous-flow culture of the ERK and L types of mammalian cells. Experimental Cell Research 33:413–421
    [Google Scholar]
  14. Stewart W. E. II, Declercq E., Billiau A., Desmyter J., Desomer P. 1972; Increased susceptibility of cells treated with interferon to the toxicity of polyriboinosinic-polyribocytidylic acid. Proceedings of the National Academy of Sciences of the United States of America 69:1851–1854
    [Google Scholar]
  15. Vilcek J., NG M. H. 1971; Post-transcriptional control of interferon synthesis. Journal of Virology 7:588–594
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-20-1-29
Loading
/content/journal/jgv/10.1099/0022-1317-20-1-29
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error