Steady-state chemostat cultures of mouse LS-cells grown in a chemically defined, protein-free medium were induced to produce interferon by a mycophage double-stranded RNA in a system potentiated by DEAE-dextran. The induction was terminated by the addition of heparin. The kinetics of interferon production in glucose-limited cultures were similar to those produced by the same induction system in batch culture. A maximum titre of approximately 2.8 log units/ml was reached 7 to 8 h postinduction. Interferon production was unaffected by changes in the cell growth rate within the range of dilution rates 0.25 to 0.35 day. Glucose-limited chemostat cultures (0.5 mg glucose/ml) yielded higher titres of interferon than cultures with excess glucose. Repeated induction of interferon was obtained in the chemostat without the development of a refractory state, although the repeated treatment was associated with inhibition of cell growth.


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