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Satellite Tobacco Necrosis Virus is the smallest virus known to date. It has a mean diameter of 180 Å with icosahedral symmetry which was determined from shadow-cast and negatively contrasted specimens. Immunological complexes of virus and anti-virus (IgG) were freed from non-specific IgG by particle sieve chromatography. The individual antibody molecules could be detected by electron microscopy in the small complexes formed with slight antigen excess. IgG molecules either bridged adjacent virus particles by their ends or were combined to a single virus particle by one of its ends, thus radiating out from the virus capsid. A few subunits could be discerned on the IgG molecules arranged in different structural configurations.
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