Growth of animal cells in a nutritionally complete tissue culture medium is usually optimal when the medium is buffered at a pH in the range 7.2 to 7.4. To function most efficiently the pKa of the chosen buffer should be as close to the required pH as possible. The most commonly used buffer in tissue culture media is the bicarbonate + carbon dioxide buffer but phosphate or tris buffers have also been used. Each of these buffers has particular disadvantages for use with biological systems. The bicarbonate + CO buffer, pKa of 6.3 at 37°, has the obvious limitation that one component is in the gaseous phase and tissue cultures must be maintained in a closed system. Although the phosphate buffer has a more suitable pKa, 6.9 at 37°, phosphates form insoluble complexes with essential divalent cations when used at concentrations necessary for a suitable buffering capacity.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error