1887

Abstract

Summary

Poliovirus mutants were cultivated at a temperature (39·6°) too high for their optimal growth but low enough to permit full growth of the parental strain. Tests for production of infectious RNA and serum-blocking antigen showed a gradient of mutant productivity that corresponded closely with an earlier physiological classification of these mutants. No mutant produced fully wild-type yields of either RNA or antigen. In general, group A mutants produced the least RNA and antigen (some yields being undetectable or < 1 % of values) while group D mutants produced the most (up to 88 % of RNA and 27·6 % of antigen). Mutants of groups B and C produced intermediate yields. Six of the mutants (all that were classified belonging to groups A or B) converted their small yield of RNA and antigen to infective virus with an efficiency not significantly different from that of wild-type virus. The remainder (thirteen mutants, most of those classified belonging to groups C or D) matured either or both their RNA and antigen inefficiently and accordingly exhibited a defect in some stage of maturation or assembly.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-2-2-297
1968-03-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/2/2/JV0020020297.html?itemId=/content/journal/jgv/10.1099/0022-1317-2-2-297&mimeType=html&fmt=ahah

References

  1. Ames B., Hartman P. E. 1963; The histidine operon. Cold Spring Harb. Symp. quant. Biol 28:349
    [Google Scholar]
  2. Cooper P. D. 1961; An improved agar cell-suspension plaque assay for poliovirus: some factors affecting efficiency of plating. Virology 13:153
    [Google Scholar]
  3. Cooper P. D. 1964; The mutation of poliovirus by 5-fluorouracil.. Virology 22:186
    [Google Scholar]
  4. Cooper P. D. 1967; A genetic map of poliovirus temperature-sensitive mutants. In preparation
    [Google Scholar]
  5. Cooper P. D., Johnson R. T., Garwes D. J. 1966; Physiological characterization of heat defective poliovirus mutants: preliminary classification. Virology 30:638
    [Google Scholar]
  6. Demars R. I. 1955; The production of phage-related materials when bacteriophage development is interrupted by proflavine. Virology 1:83
    [Google Scholar]
  7. Fraenkel-Conrat H., Singer B., Tsugita A. 1961; Purification of viral RNA by means of bentonite. Virology 14:54
    [Google Scholar]
  8. Jacob F., Monod J. 1961; On the regulation of gene activity. Cold Spring Harb. Symp. quant. Biol 26:193
    [Google Scholar]
  9. Pagano J. S., Vaheri A. 1965; Enhancement of infectivity of poliovirus RNA with diethylammo- ethyl-dextran (DEAE-D). Arch. ges. Virusforsch 17:456
    [Google Scholar]
  10. Wilson J. N., Cooper P. D. 1963; Aspects of the growth of poliovirus as revealed by the photodynamic effects of neutral red and acridine orange. Virology 21:135
    [Google Scholar]
  11. Wilson J. N., Cooper P. D. 1965; The effect of light on poliovirus grown in neutral red. Virology 26:1
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-2-2-297
Loading
/content/journal/jgv/10.1099/0022-1317-2-2-297
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error