@article{mbs:/content/journal/jgv/10.1099/0022-1317-2-2-243, author = "Zwartouw, H. T. and Algar, D. J.", title = "Growth of High-titre Semliki Forest Virus in Concentrated Suspensions of Chick Embryo Cells", journal= "Journal of General Virology", year = "1968", volume = "2", number = "2", pages = "243-250", doi = "https://doi.org/10.1099/0022-1317-2-2-243", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-2-2-243", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY Freshly prepared chick embryo cells have a lower glycolysis rate than cultured cells and can be used at higher concentrations in suspension to produce virus. Conditions for producing maximal yields (about 103 p.f.u./ cell) of Semliki Forest virus from suspensions containing 107 to 108 cells/ml. were: (1) suitable preparation of the cells, (2) incubation at 31°, (3) maintenance of pH at 7 to 7·4, (4) adequate glucose, (5) adequate oxygen, (6) gentle agitation. The cultures required no nutrient other than glucose and the cells were suspended in Earle’s saline. For cell concentrations up to 3 × 107/ml. cultures were stirred in a simple sealed vessel and buffered with NaHCO3/CO2. Cultures containing 108 cells/ml. were stirred in a vessel equipped with automatic pH control and produced 1011 p.f.u./ml. of virus.", }