Freshly prepared chick embryo cells have a lower glycolysis rate than cultured cells and can be used at higher concentrations in suspension to produce virus. Conditions for producing maximal yields (about 10 p.f.u./cell) of Semliki Forest virus from suspensions containing 10 to 10 cells/ml. were: (1) suitable preparation of the cells, (2) incubation at 31°, (3) maintenance of pH at 7 to 7.4, (4) adequate glucose, (5) adequate oxygen, (6) gentle agitation. The cultures required no nutrient other than glucose and the cells were suspended in Earle's saline. For cell concentrations up to 3 × 10/ml. cultures were stirred in a simple sealed vessel and buffered with NaHCO/CO. Cultures containing 10 cells/ml. were stirred in a vessel equipped with automatic pH control and produced 10 p.f.u./ml. of virus.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error