The interaction of herpes virus particles with BSC1 cells was studied with purified virus particles labelled in the DNA and coat proteins. Intact virions adsorbed to the cell within 3 hr, a process not affected by inhibitors of nucleic acid and protein synthesis. Heparin, a negatively charged polysaccharide, interfered with the interaction of the virions with the cell membrane, and released virions attached to the cell surface. After entering the cytoplasm, some virus particles were associated with large structures. The virus particles were uncoated by existing cellular enzymes and naked viral DNA, but not the coat proteins, was gradually transported into the nuclei.
EpsteinM. A.,
HummelerK.,
BerkaloffA.1964; The entry and distribution of herpes virus and colloidal gold into HeLa cells after contact in suspension. J. exp. Med 119:291
TakemotoK. K.,
LiebhaberH.1961; Virus polysaccharide interactions. I. An agar polysaccharide determining plaque morphology of EMC virus. Virology 14:456
WatsonD. H.,
RussellW. C.,
WildyP.1963; Electron microscopic particle counts on herpes virus using the phosphotungstate negative staining technique. Virology 19:250