1887

Abstract

SUMMARY

The interaction of herpes virus particles with BSC cells was studied with purified virus particles labelled in the DNA and coat proteins. Intact virions adsorbed to the cell within 3 hr, a process not affected by inhibitors of nucleic acid and protein synthesis. Heparin, a negatively charged polysaccharide, interfered with the interaction of the virions with the cell membrane, and released virions attached to the cell surface. After entering the cytoplasm, some virus particles were associated with large structures. The virus particles were uncoated by existing cellular enzymes and naked viral DNA, but not the coat proteins, was gradually transported into the nuclei.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-2-2-231
1968-03-01
2022-07-01
Loading full text...

Full text loading...

/deliver/fulltext/jgv/2/2/JV0020020231.html?itemId=/content/journal/jgv/10.1099/0022-1317-2-2-231&mimeType=html&fmt=ahah

References

  1. Becker Y., Olshevsky U., Levitt J. 1967; The role of arginine in the replication of herpes virus. J. gen. Virol 1:471
    [Google Scholar]
  2. Benda L. 1966; Effect of heparin on B virus multiplication. in vitro. Acta virol Prague: 10:376
    [Google Scholar]
  3. Dales S. 1962; An electron microscope study of the early association between two mammalian viruses and their hosts. J. cell. Biol 13:303
    [Google Scholar]
  4. Eagle H. 1959; Amino acid metabolism in mammalian cultures. Science; N.Y: 130432
    [Google Scholar]
  5. Epstein M. A., Hummeler K., Berkaloff A. 1964; The entry and distribution of herpes virus and colloidal gold into HeLa cells after contact in suspension. J. exp. Med 119:291
    [Google Scholar]
  6. Holmes I. H., Watson D. H. 1963; An electron microscopic study of the attachment and penetration of herpes virus in BHK 21 cells. Virology 21:112
    [Google Scholar]
  7. Joklik W. K. 1964a; The intracellular uncoating of poxvirus DNA. I. The fate of radioactively labelled rabbit poxvirus. J. molec. Biol 8:263
    [Google Scholar]
  8. Joklik W. K. 1964b; The intracellular uncoating of poxvirus DNA. II. The molecular basis of the uncoating process. J. molec. Biol 8:277
    [Google Scholar]
  9. Joklik W. K. 1966; The molecular basis of the viral eclipse phase. Prog. Med. Virol 7:44
    [Google Scholar]
  10. Joklik W. K., Becker Y. 1964; The replication and coating of vaccinia virus DNA. J. molec. Biol 10:452
    [Google Scholar]
  11. Joklik W. K., Darnell J. E. 1961; Adsorption and early fate of purified poliovirus in HeLa cells. Virology 13:439
    [Google Scholar]
  12. Levitt J., Becker Y. 1967; The effect of cytosine arabinoside on the replication of herpes simplex virus. Virology 31:129
    [Google Scholar]
  13. Morgan C., Rose H. M., Holden M., Jones E. P. 1959; Electron microscope observations on the development of herpes simplex virus. J. exp. Med 110:643
    [Google Scholar]
  14. Nahmias A. J., Kibrick S. 1964; Inhibitory effect of heparin on herpes simplex virus. J. Bact 87:1060
    [Google Scholar]
  15. Olshevsky U., Levitt J., Becker Y. 1967; Studies on the synthesis of herpes simplex virions. Virology 33:323
    [Google Scholar]
  16. Takemoto K. K., Fabish P. 1964; Inhibition of herpes virus by natural and synthetic polysaccharides. Proc. Soc. exp. Biol. Med 116:140
    [Google Scholar]
  17. Takemoto K. K., Liebhaber H. 1961; Virus polysaccharide interactions. I. An agar polysaccharide determining plaque morphology of EMC virus. Virology 14:456
    [Google Scholar]
  18. Vaheri A., Cantell K. 1963; The effect of heparin on herpes simplex virus. Virology 21:661
    [Google Scholar]
  19. Warner J. R., Knopf P., Rich A. 1963; A multiple ribosomal structure in protein synthesis. Proc. natn. Acad. Sci., U.S.A 49:122
    [Google Scholar]
  20. Watson D. H., Russell W. C., Wildy P. 1963; Electron microscopic particle counts on herpes virus using the phosphotungstate negative staining technique. Virology 19:250
    [Google Scholar]
  21. Wildy P., Watson D. H. 1962; Electron microscopic studies on the architecture of animal viruses. Cold Spring Harb. Symp. quant. Biol 27:25
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-2-2-231
Loading
/content/journal/jgv/10.1099/0022-1317-2-2-231
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error