1887

Abstract

SUMMARY

Interaction of -chloromercuribenzoate with the haemagglutinins of adenovirus types 3, 4 and 7 resulted in the loss of their ability to agglutinate red cells—a loss which can be either reversible or irreversible. The structural integrity of the haemagglutinins of types 3 and 7 was maintained in the process of their reversible inactivation in 0·2 -tris buffer, but inactivation was irreversible if they were allowed to react with -chloromercuribenzoate in 0·1 -borate buffer (pH 8·0) or if the reversibly inactivated haemagglutinins were transferred into the borate buffer even in the absence of the mercurial compound. Thus the reversibly inactivated haemagglutinins are predisposed to irreversible loss of activity. Haemagglutinin of type 4 was irreversibly inactivated in both buffers. Virus particles of adenovirus types 3, 4 and 7 are degraded by treatment with -chloromercuribenzoate in the borate buffer.

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1968-03-01
2021-10-28
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References

  1. Allison A. C., Buckland F. E., Andrewes C. H. 1962; Effects of sulfhydryl reagents on infectivity of some viruses. Virology 17:171
    [Google Scholar]
  2. Barnard E. A., Stein W. D. 1958; The roles of imidazole in biological systems. Adv. Enzymol 20:51
    [Google Scholar]
  3. Brewer C. F., Riehm J. P. 1967; Evidence for possible non-specific reactions between V-ethyl-maleimide and proteins. Analyt. Biochem 18:248
    [Google Scholar]
  4. Buckland F. E. 1960; Inactivation of virus hacmagglutinins by para-chloromercuribenzoic acid. Nature; Lond: 188768
    [Google Scholar]
  5. Cowan K. M. 1966; Effect of merthiolate on agar gel diffusion precipitin reactions with foot-and- mouth disease virus. J. Immun 97:647
    [Google Scholar]
  6. Gerhart J. C., Schachman H. K. 1965; Distinct subunits for the regulation and catalytic activity of aspartate transcarbamylase. Biochemistry 4:1054
    [Google Scholar]
  7. Godschalk W., Veldstra H. 1965; Investigations on turnip yellow mosaic virus interactions with aliphatic mercurials. I. Archs Biochem. Biophys 111:161
    [Google Scholar]
  8. Himes R. H., Rabinowitz J. C. 1962; Formyltetrahydrofolate synthetase. II. Characteristics of the enzyme and the enzymic reaction. J. biol. Chem 237:2903
    [Google Scholar]
  9. Kaper J. M., Houwing C. 1962a; Studies on the interaction ofp-chloromercuribenzoate with turnip yellow mosaic virus and with its artificial top component. I. Spectrophotometric measurement of the interaction and some properties of the reaction products. Archs Biochem. Biophys 96:125
    [Google Scholar]
  10. Kaper J. M., Houwing C. 1962b; Studies on the interaction ofp-chloromercuribenzoate with turnip yellow mosaic virus and with its artificial top component. II. Interrelationships of the interaction and structural changes. Archs Biochem. Biophys 97:449
    [Google Scholar]
  11. Kaper J. M., Jenifer F. G. 1965; Studies on the interaction of p-chloromercuribenzoate with turnip yellow mosaic virus. III. Involvement of the ribonucleic acid. Archs Biochem. Biophys 112:331
    [Google Scholar]
  12. Kaper J. M., Jenifer F. G. 1967; Studies on the interaction of p-mercuribenzoate with turnip yellow mosaic virus. IV. Conformational change, exposure of buried prototropic groups, and pH-induced degradation. Biochemistry 6:440
    [Google Scholar]
  13. Keresztes-Nagy S., Klotz I. M. 1963; Mercaptan involvement in dissociation and reconstitution of hemerythrin. Biochemistry 2:923
    [Google Scholar]
  14. Madsen N. B., Corn C. F. 1956; The interaction of muscle phosphorylase with p-chloromercuri- benzoate. I. Inhibition of activity and effect on the molecular weight. J. biol. Chem 223:1055
    [Google Scholar]
  15. Martin R. G., Ames B. N. 1961; A method for determining the sedimentation behavior of enzymes: Application to protein mixtures. J. biol. Chem 236:1372
    [Google Scholar]
  16. Neurath A. R. 1965; Fluorometric estimation of antigens (antibodies). Z. Naturf 20b:974
    [Google Scholar]
  17. Neurath A. R., Rubin B. A., Vernon S. K. 1966; Quantitative determination of adenovirus antigens by means of a fluorescent precipitin test. Experientia 22:653
    [Google Scholar]
  18. Norrby E. 1966a; The relationship between the soluble antigens and the virion of adenovirus type 3. I. Morphological characteristics. Virology 28:236
    [Google Scholar]
  19. Norrby E. 1966b; The relationship between the soluble antigens and the virion of adenovirus type 3. II. Identification and characterization of an incomplete haemagglutinin. Virology 30:608
    [Google Scholar]
  20. Norrby E., Wadeix G. 1967; Soluble components of adenovirus type 4. Virology 31:592
    [Google Scholar]
  21. Pecci J., Fujimori E. 1967; Mercurial-induced dissociation of phycoerythrin from Ceramium rubrum . Biochim. biophys. Acta 131:147
    [Google Scholar]
  22. Philipson L. 1964; Degradation of polio virions by mercurials. Biochem. biophys. Res. Commun 17:352
    [Google Scholar]
  23. Polasa H., Green M. 1967; Adenovirus proteins. I. Amino acid composition of oncogenic and non-oncogenic human adenoviruses. Virology 31:565
    [Google Scholar]
  24. Reichmann M. E., Hatt D. L. 1961; The effect of the substitution of sulfhydryl groups on the macromolecular structure of Potato Virus X. Biochim. biophys. Acta 49:153
    [Google Scholar]
  25. Rosemeyer M. A., Huehns E. R. 1967; On the mechanism of the dissociation of haemoglobin. J.molec. Biol 25:253
    [Google Scholar]
  26. Sever J. L. 1962; Application of a microtechnique to viral serological investigations. J. Immun 88:320
    [Google Scholar]
  27. Tamaoki T., Miyazawa F. 1967; Dissociation ofEscherichia coliribosomes by sulfhydryl reagents. J. molec. Biol 23:35
    [Google Scholar]
  28. Wang J. H. -C., Matheson A. T. 1967; The interaction ofEscherichia coliribosomes with p-chloromercuribenzoate. Biochem. biophy. Acta 138:296
    [Google Scholar]
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