Full text loading...
A simple and rapid in vitro labelling technique is described for preparing tobacco mosaic virus labelled with 125I. The technique is versatile and has been used to obtain labelled virus of both high and low specific activity. High specific activity virus has been used in a pilot radioautographic experiment at the electron microscope level, and micrographs were obtained showing virus particles in close association with silver grains. At specific activities of 2 to 3 mc/mg. a rapid loss of infectivity was observed after labelling; this effect was not observed when the specific activity was 1 µc/mg. The sensitivity of detection for high specific activity preparations by scintillation counting and by electron microscope radioautography is discussed and the potentiality of 12SI-labelled virus of both high and low specific activity is indicated.
Article metrics loading...
Full text loading...
References
Data & Media loading...