RT Journal Article SR Electronic(1) A1 Salim, A. R.YR 1968 T1 Conditions for Plaque Formation with a Phlebotomus (Sandfly) Fever Virus JF Journal of General Virology, VO 2 IS 1 SP 81 OP 87 DO https://doi.org/10.1099/0022-1317-2-1-81 PB Microbiology Society, SN 1465-2099, AB Summary Naples virus, a member of the Phlebotomus fever group of arboviruses, is able to multiply in cells derived from hamster embryos. It produces no cyto- pathic effect in these cells in a fluid medium and no plaques upon infection of confluent monolayers overlaid with a medium containing agar or the sodium salt of carboxymethylcellulose. Plaques are produced only when these cells are infected in suspension and plated to form monolayers under an overlay containing carboxymethylcellulose. A critical number of cells is required and the presence of carboxymethylcellulose is essential. The survival or death of a hamster embryo cell infected with this virus appears to depend upon the stage of growth of the cells at the time of infection and the environmental conditions., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-2-1-81