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Conditions for Plaque Formation with a Phlebotomus (Sandfly) Fever Virus
Abstract
Summary
Naples virus, a member of the Phlebotomus fever group of arboviruses, is able to multiply in cells derived from hamster embryos. It produces no cyto- pathic effect in these cells in a fluid medium and no plaques upon infection of confluent monolayers overlaid with a medium containing agar or the sodium salt of carboxymethylcellulose. Plaques are produced only when these cells are infected in suspension and plated to form monolayers under an overlay containing carboxymethylcellulose. A critical number of cells is required and the presence of carboxymethylcellulose is essential. The survival or death of a hamster embryo cell infected with this virus appears to depend upon the stage of growth of the cells at the time of infection and the environmental conditions.
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© Society for General Microbiology 1968