1887

Abstract

Summary

RNA was extracted from purified Sendai virus, labelled with P, and sedimented in sucrose density gradients. Under conditions of high ionic strength (0·1 -NaCl) the sedimentation coefficient was 57 In the presence of 0·005 -EDTA, the sedimentation coefficient was reduced to 40 indicating that the RNA of the virus exists as a single-stranded molecule.

The intracellular synthesis of virus-directed RNA during the latent period of infection of chick embryo cells by Sendai virus in the presence of actino- mycin was also studied. Cells were extracted by the phenol + sodium dodecyl sulphate method at 2,4, 6 and 7 hr after infection, having previously been exposed to [H]uridine for at least 2 hr. It was found that the first newly formed RNA had a sedimentation coefficient of 18 S' and could be detected between 2 and 4 hr after infection. From 4 hr onwards a 57 component was found in increasing amounts until 7 hr after infection. Substantially similar results were obtained when infected cells were treated with detergent alone, and the mixture immediately centrifuged on density gradients except that the principal RNA components now had sedimentation values of 57 and 23

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1968-01-01
2022-01-22
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References

  1. Barry R. D. 1965; Factors which affect the release of Newcastle disease and Sendai viruses from infected allantoic cells. J. gen. Microbiol 39:229
    [Google Scholar]
  2. Barry R. D., Davies P. 1968; The sedimentation of influenza virus RNA in sucrose density gradients. J. gen. Virol 2:59
    [Google Scholar]
  3. Boedtker H. 1960; Configurational properties of tobacco mosaic virus RNA. J. molec. Biol 2:171
    [Google Scholar]
  4. Bratt M. A., Robinson W. S. 1967; Ribonucleic acid synthesis in cells infected with Newcastle disease virus. J. molec. Biol 23:1
    [Google Scholar]
  5. Bray G. A. 1960; A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Anal. Biochem 1:279
    [Google Scholar]
  6. Bukrinskaya A. G., Vladimircava E. A., Gitelman A. K., Smirnov Y. A. 1966; Myxovirus induced early RNA and early proteins. Abst. IX Int. Congr. Microbiol Moscow: p 494
    [Google Scholar]
  7. Davies P., Barry R. D. 1966; Nucleic acid of influenza virus. Nature; Lond: 211384
    [Google Scholar]
  8. Duesberg P., Robinson W. S. 1965; Isolation of the nucleic acid of Newcastle disease virus (NDV). Proc. natn. Acad. Sci. U.S.A 54:794
    [Google Scholar]
  9. Iwai Y., Iwai M., Okumoto M., Hosokawa T., Asai T. 1966; Properties of the nucleic acid isolated from HVJ. Biken. J 9:241
    [Google Scholar]
  10. Kingsbury D. W. 1966; Newcastle disease virus RNA. 1. Isolation and preliminary characterization of RNA from virus particles. J. molec. Biol 18:195
    [Google Scholar]
  11. Martin R. G., Ames B. N. 1961; A method for determining the sedimentation behaviour of enzymes: application to protein mixtures. J. Biol. Chem 336:1372
    [Google Scholar]
  12. Prince A. M., Adams W. R. 1966; Characteristics of rapidly sedimenting ribonucleic acid from Harris strain Rous sarcoma virus and infected cells. Virology 30:151
    [Google Scholar]
  13. Robinson W. S., Pitkanen A., Rubin H. 1965; The nucleic acid of the Bryan strain of Rous sarcoma virus: purification of the virus and isolation of the nucleic acid. Proc. natn. Acad. Sci. U.S.A 54:137
    [Google Scholar]
  14. Tikchonenko T. I., Kisseuov F. L., Bukrinskaya A. G., Smirnov Y. 1964; Secondary structure of RNA from Sendai virus. Nature; Lond: 2021363
    [Google Scholar]
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