1887

Abstract

SUMMARY

One hundred and eighteen different strains of were investigated for bacteriocinogeny. These and an additional 44 strains of were used as indicators. Sixty-seven of the strains had a nontransmissible killing effect on one or more of the indicator organisms and 30 of these 67 bacteriocins with different spectra of activity were further investigated. Individual bacteriocins killed from 5 to 87 of the indicators and a number of 44 different strains but had no action on strains of other species of the family . Broth cultures of bacteriocinogenic strains are inducible by ultraviolet light and yield bacterio- cin titres of about 1/100. Activity is sedimentable by high-speed centrifugation. Electron microscopy of all 30 preparations revealed similar phage-tail-like structures with a contractile sheath round a hollow core. The structures consisted of protein and did not contain DNA. The particles resembled some pyocins and also the tail of a transducing phage. In 2 preparations a few phage-like particles resembling other Proteus phages were also seen. Bacteriocin activity was always associated with uncontracted sheaths, and triggered tails did not adsorb to susceptible organisms. We conclude that the tail-like structures are the products of defective lysogeny. The high incidence of the latter state may be accounted for by the selection of genes favourable to the host which were originally acquired through transduction by lysogenization or lysogenic conversion.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-2-1-29
1968-01-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/2/1/JV0020010029.html?itemId=/content/journal/jgv/10.1099/0022-1317-2-1-29&mimeType=html&fmt=ahah

References

  1. Abbott J. D., Shannon R. 1958; A method for typing Shigella sonnei, using colicine production as a marker. J. clin. Path 11:71
    [Google Scholar]
  2. Arber W., Kellenberger G. 1958; Studies of the properties of seven defective-lysogenic strains derived from Escherichia coli k 12 (λ). Virology 5:458
    [Google Scholar]
  3. Bradley D. E. 1962; A study of the negative staining process. J. gen. Microbiol 29:503
    [Google Scholar]
  4. Bradley D. E., Dewar C. A. 1966; The structure of phage-like objects associated with noninduced bacteriocinogenic bacteria. J. gen. Microbiol 45:399
    [Google Scholar]
  5. Brenner S., Horne R. W. 1959; A negative staining method for high resolution electron microscopy of viruses. Biochim. biophys. Acta 34:103
    [Google Scholar]
  6. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J 62:315
    [Google Scholar]
  7. Campbell A. 1961; Conditions for the existence of bacteriophage. Evolution 15:153
    [Google Scholar]
  8. Coetzee J. N. 1961; Lysogenic conversion in the genus Proteus. Nature; Lond: 189946
    [Google Scholar]
  9. Coetzee J. N. 1963; Lysogeny in Proteus rettgeri and the host-range of P. rettgeri and P. hauseri bacteriophages. J. gen. Microbiol 31:219
    [Google Scholar]
  10. Coetzee J. N. 1967; Bacteriocinogeny in strains of Providence and Proteus morganii. Nature; Lond: 213614
    [Google Scholar]
  11. Coetzee J. N., Sacks T. G. 1960; Lysogeny in the genus Proteus. 1. Incidence of lysogenic strains. S. Afr. J. lab. clin. Med 6:123
    [Google Scholar]
  12. Coetzee J. N., De Klerk H. C., Smit J. A. 1967; A transducing bacteriophage for Proteus vulgaris. J. gen. Virol 1:561
    [Google Scholar]
  13. Coetzee J. N., Smit J. A., Prozesky O. W. 1966; Properties of Providence and Proteus morganii transducing phages. J. gen. Microbiol 44:167
    [Google Scholar]
  14. Cradock-Watson J. E. 1965; The production of bacteriocines by Proteus species. Zentbl. Bakt. Orig. 1 196:385
    [Google Scholar]
  15. De Klerk H. C., Smit J. A. 1967; Properties of a Lactobacillus fermenti bacteriocin. J. gen. Microbiol 48:309
    [Google Scholar]
  16. Dienes L. 1946; Reproductive processes in Proteus cultures. Proc. Soc. exp. Biol. N.Y 63:265
    [Google Scholar]
  17. Dische Z. 1953; Qualitative and quantitative colorimetric determination of heptoses. J. biol Chem 204:983
    [Google Scholar]
  18. Endo H., Ayabe K., Amako K., Takeya K. 1965; Inducible phage of Escherichia coli 15. Virology 25:469
    [Google Scholar]
  19. Herriot R. M. 1951; Nucleic-acid-free T2 virus ‘ghosts’ with specific biological action. J. Bact 61:752
    [Google Scholar]
  20. Hughes W. H. 1957; A reconsideration of the swarming of Proteus vulgaris. J. gen. Microbiol 17:49
    [Google Scholar]
  21. Ishn S., Nishi Y., Egami F. 1965; The fine structure of a pyocin. J. molec. Biol 13:428
    [Google Scholar]
  22. Kellenberger E., Arber W. 1955; Die Struktur des Schwanzes der Phagen T2 und T4 und der Mechanismus der irreversiblen Adsorption. Z. Naturf 10b:698
    [Google Scholar]
  23. Kellenberger E., Séchaud J. 1957; Electron microscopical studies of phage multiplication. 2. Production of phage-related structures during multiplication of phages T2 and T4. Virology 3:256
    [Google Scholar]
  24. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with with the Folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  25. Maré I. J., Coetzee J. N., De Klerk H. C. 1964; Agar electrophoresis of colicines with an Alcaligenes faecalis indicator strain. Nature; Lond: 202213
    [Google Scholar]
  26. Neubauer Z. 1967; Brief consideration of the meaning of lysogenic conversion in Salmonella anatum phage system. Nature; Lond: 2131263
    [Google Scholar]
  27. Prozesky O. W., De Klerk H. C., Coetzee J. N. 1965; The morphology of Proteus bacteriophages. J. gen. Microbiol 41:29
    [Google Scholar]
  28. Reeves P. 1965; The bacteriocins. Bact. Rev 29:24
    [Google Scholar]
  29. Sandoval H. K., Reilly H. C., Tandler B. 1965; Colicin 15: Possibly a defective bacteri- phage. Nature; Lond: 205522
    [Google Scholar]
  30. Scott T. A., Melvin E. H. 1953; Determination of dextran with anthrone. Analyt. Chem 25:1656
    [Google Scholar]
  31. Seaman E., Tarmy E., Marmur J. 1964; Inducible phages of Bacillus subtilis. Biochemistry 3:607
    [Google Scholar]
  32. Smit J. A., Coetzee J. N. 1967; Serological specificities of phenylalanine deaminases of the Proteus-Providence group. Nature; Lond: in the Press
    [Google Scholar]
  33. Stickler D. J., Tucker R. G. 1967; Studies on the infection of Bacillus subtilis with a defective bacteriophage. J. gen. Microbiol 46:vii
    [Google Scholar]
  34. Takeya K., Minamishima Y., Amako K., Ohnishi Y. 1967; A small rod-shaped pyocin. Virology 31:166
    [Google Scholar]
  35. Taubeneck U. 1963; Über die Produktion biologische aktiver Phagen-Schwänze durch einen defekt lysogenen Proteus mirabilis-Stamm. Z. Naturf 18b:989
    [Google Scholar]
  36. Van Iterson W., Hoeniger J. F. M., Nijman Van Zanten E. 1967; A microtubule in a bacterium. J. Cell Biol 32:1
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-2-1-29
Loading
/content/journal/jgv/10.1099/0022-1317-2-1-29
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error