1887

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Volume 2, Issue 1

Role of Adenovirus Antigens in the Induction of Virus Neutralizing Antibody Free

Abstract

SUMMARY

Guinea pig antisera were prepared against purified hexon, penton and fibre antigens of adenovirus type 5. Sera reacting specifically with particular antigens as judged by immunodiffusion, complement fixation and haemagglu- tination-inhibition tests were assayed for virus neutralizing capacity. The latter was exerted almost exclusively by anti-hexon sera. Type 5 anti-hexon but not anti-fibre sera neutralized type 1 virus provided the serum virus reaction took place at low pH values.

Since the hexon antigens contain the group-specific adenovirus antigen, demonstrable by complement fixation or immunodiffusion, the ability of heterotypic adenovirus rabbit antisera to combine with adenovirus type 5 was assayed. Evidence of such combinations was obtained by the demonstration of virus inactivation following addition of a goat anti-rabbit serum. It was shown that adenovirus type 1 and type 2 antisera combined with type 5 virus. The technique used did not reveal combinations between type 5 virus and antisera against type 3, type 12, type 21, nor against Cox- sackie B. The IgG fractions of rabbit antisera against type 1, type 2 and type 12 were shown to enhance inactivation of type 5 virus+antibody complexes. No enhancement was obtained with type 3 or Coxsackie B, IgG. The results suggest that although the hexon antigens contain common structures the configurational pattern differs among the different virus types.

  • Received:
  • Accepted:
  • Published Online:
© Society for General Microbiology 1968
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/content/journal/jgv/10.1099/0022-1317-2-1-177
1968-01-01
2023-09-29
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Role of Adenovirus Antigens in the Induction of Virus Neutralizing Antibody
J. Gen. Virol. 2, 177 (1968); https://doi.org/10.1099/0022-1317-2-1-177
/content/journal/jgv/10.1099/0022-1317-2-1-177
/content/journal/jgv/10.1099/0022-1317-2-1-177
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