1887

Abstract

Summary

The incorporation of [C]amino acids into the acid precipitable material of chick embryo fibroblasts was inhibited by both sparsomycin and gougero- tin with an efficiency which varied according to the amino acid and to the antibiotic. Of 16 amino acids tested, proline was the most susceptible to the action of sparsomycin and phenylalanine was the most resistant. With gougerotin, the order of decreasing susceptibilities of four amino acids were: phenylalanine, proline and glycine, lysine. Inhibition of virus growth by the two antibiotics was also specific. Synthesis of pseudorabies virus was very susceptible to inhibition by sparsomycin, while vaccinia virus synthesis was almost completely resistant. Newcastle disease virus, fowl plague and Western equine encephalomyelitis viruses showed intermediate susceptibilities to that drug.

In contrast, the synthesis of fowl plague virus was much more inhibited by gougerotin than that of Newcastle disease virus, and this antibiotic had an equal action on the growth of pseudorabies and vaccinia viruses. Most of the results may be explained by assuming that sparsomycin is an effective inhibitor of the activity of virus messenger RNAs which are rich in guanine and cytosine, while gougerotin is more effective on uracil-rich virus messenger RNAs.

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/content/journal/jgv/10.1099/0022-1317-2-1-143
1968-01-01
2022-01-17
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References

  1. Bratt M. A., Robinson W. S. 1967; Ribonucleic acid synthesis in cells infected with Newcastle disease virus. J. molec. Biol 23:1
    [Google Scholar]
  2. Casjens S. R., Morris A. J. 1965; The selective inhibition of protein assembly by gougerotin. Biochim. biophys. Acta 108:677
    [Google Scholar]
  3. Duesberg P. H., Robinson W. S. 1967; On the structure and replication of influenza virus. J. molec. Biol 25:383
    [Google Scholar]
  4. Goldberg I., Mitsugi K. 1966; Sparsomycin, an inhibitor of aminoacyl transfer to polypeptide. Biochem. biophys. Res. Commun 23:453
    [Google Scholar]
  5. Goldberg I., Mrrsugi K. 1967; Sparsomycin inhibition of polypeptide synthesis promoted by synthetic and natural polynucleotides. Biochemistry 6:372
    [Google Scholar]
  6. Kingsbury D. W. 1966; Newcastle disease virus RNA. II. Preferential synthesis of RNA complementary to parental viral RNA by chick embryo cells. J. molec. Biol 18:204
    [Google Scholar]
  7. Sprecher-Goldberger S. 1964; A differential effect of ethidium chloride on the growth of Arbo A viruses and of their small plaque mutants. Acta virol 8:80
    [Google Scholar]
  8. Sprecher-Goldberger S. 1966; The existence of two different metabolic patterns for the synthesis of RNA viruses. Arch. Virusforsch 18:198
    [Google Scholar]
  9. Subak-Sharpe H., Bürk R. R., Crawford L. V., Morrison J. M., Hay J., Keir H. M. 1966; An approach to evolutionary relationships of mammalian DNA viruses through analysis of the pattern of nearest neighbour base sequences. Cold Spring Harb. Symp. quant. Biol 31:737
    [Google Scholar]
  10. Woodson B. 1967; Vaccinia m-RNA synthesis under conditions which prevent uncoating. Biochem. biophys. Res. Commun 27:169
    [Google Scholar]
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